接种量及蔗糖浓度对悬浮培养玫瑰茄细胞生长的影响

以悬浮培养玫瑰茄细胞为研究对象，探讨了不同初始接种量以及蔗糖浓度对玫瑰茄细胞生长的影响。结果表明随着接种量增大，细胞生长启动加快，但细胞比生长速率却逐渐降低，这表明细胞的分裂启动存在着密度依赖现象，而细胞的生长却存在密度抑制现象；在ρ（蔗糖）为２０～５－ｇ／Ｌ范围内，当ρ（蔗糖）为４０ｇ／Ｌ时，蔗糖是作为限制性基质而存在，而当ρ（蔗糖）超过４０ｇ／Ｌ时，蔗糖表现出基质抑制效应，这明显体现在玫瑰茄细

The Effects of Inoculum Amount and Sucrose Concentration on the Growth of Suspension Roselle Cell

The suspension roselle cell was used to study the effects of inoculum amount and sucrose concentration on its growth. The results were as follows: the bigger the inoculum amount was, the faster the roselle cell started to grow, but the smaller the specific growth rate was. From these facts, the density dependent of cell starting to grow and density inhibited of cell growth were proposed; Sucrose was limiting substrate from 2% to 4%; But from 4% to higher concentration, the sucrose showed inhibition effect on cell growth. The dual effects of sucrose on cell growth were apparently seen from the utilization of nutrients ,such as sugar and nitrate.