| 人参锈腐病拮抗放线茵的鉴定及发酵条件 |
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| 引用本文： | 姜云,陈长卿,王琳,马贵龙.人参锈腐病拮抗放线茵的鉴定及发酵条件[J].东北林业大学学报,2011(9):101-104. |
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| 作者姓名： | 姜云 陈长卿 王琳 马贵龙 |
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| 作者单位： | 吉林农业大学 |
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| 基金项目： | 吉林省教育厅项目(2008-307);吉林农业大学科研启动基金项目(201007) |
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| 摘 要： | 从人参田土壤中筛选到1株对人参锈腐病菌(Cylindrocarpon destructans)有较强拮抗作用的菌株1a-3,通过形态特征、培养特性、生理生化特性、细胞壁组分分析和16S rDNA同源性序列分析,确定该菌株为链霉菌属灰产色链霉菌(Streptomyces griseochromogenes).经单因子和各...
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| 关 键 词： | 人参锈腐病 拮抗菌 放线菌 发酵条件 |
Identification of an Actinomycete Antagonistic Against Cylindrocarpon destructans and Its Optimized Fermentation Conditions |
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| Jiang YunCollege of Life Sciences,Jilin Agricultural University,Changchun,P.R.China;Chen Changqing,Wang Lin,Ma Guilong.Identification of an Actinomycete Antagonistic Against Cylindrocarpon destructans and Its Optimized Fermentation Conditions[J].Journal of Northeast Forestry University,2011(9):101-104. |
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| Authors: | Jiang YunCollege of Life Sciences Jilin Agricultural University Changchun PRChina;Chen Changqing Wang Lin Ma Guilong |
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| Institution: | (College of Agronomy,Jilin Agricultural University) |
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| Abstract: | An actinomycete strain 1a-3 antagonistic against Cylindrocarpon destructans was isolated from the soil in ginseng fields.It was identified as Streptomyces griseochromogenes according to the morphological characteristics,cultural characteristics,physiological and biochemical properties,cell wall components and 16S rDNA sequences analysis.Single factor experiment and multiple factor experiment showed that the culture medium which contained 1g/L glucose,3g/L corn flour,2g/L soybean flour,0.3g/L yeast flour,0.1g/L KH2PO4,0.3g/L CaCO3 and 0.1g/L NaCl was the best for fermentation.The optimal fermentation condition was obtained as initial pH 7.0,fermentation temperature 28 degrees C,and fermentation time 60h. |
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| Keywords: | Actinomycete Antagonistic bacteria Fermentation conditions |
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