金线莲丛生芽诱导研究

[目的]为提高金线莲的繁殖系数。[方法]以金线莲为材料,以MS为基本培养基,设置不同的激素组合,进行分化增殖培养,定期观察并记录结果。[结果]升汞浓度和消毒时间是金线莲无菌体系建立的重要影响因素。不同消毒方式对金线莲启动培养的污染率极显著差异,褐变率有显著差别。金线莲启动培养最佳消毒方式为:75%酒精消毒30 s+0.15%升汞消毒4 min。不同激素浓度及配比对丛生芽诱导的影响差异极显著。培养基中附加6-BA 1.0 mg/L+2,4-D 0.10 mg/L较其他培养基对丛生芽诱导有极显著差异,增殖1.166 7倍。筛选出的丛生芽诱导最佳培养基为MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L+3%蔗糖+7 g/L琼脂。[结论]得到了适宜的诱导金线莲丛生芽的培养基。

Induction of Adventitious Buds of Anoectochilus roxburghii

[Objective] The aim was to improve the propagation coefficient of Anoectochilus roburghii.[Method] With A.roburghii as material and MS medium as basic medium,different combinations of hormone were set up for differentiation and propagation culture.The regular observation was made and the result was recorded. [Result] The chloride hydrargyrum concn.and disinfection time were the main influencing factors for the establishment of aseptic system of A.roburghii.The contamination rate of different disinfection methods for the initiation culture of A.roburghii showed a very significant difference and its browning rate had a very significant difference.The optimal disinfection method for the initiation culture of A.roburghii was disinfection with 75% ethanol for 30 s+0.15% chloride hydrargyrum for 4 min.The effects of different hormone concn.and their combinations on the induction of adventitious buds showed a very significant difference.Medium supplemented with 6-BA 1.0 mg/L+2,4-D 0.10 mg/L had a very significant difference with other mediums and the proliferation was 1.166 7 times. The optimum medium screened out for adventitious buds was MS+6-BA1.0 mg/L+2,4D 0.1 mg/L+3% saccharose+7 g/L agar.[Conclusion] The optimum medium for the induction of A.roburghii adventitious buds was obtained.