| 转基因抗草丁膦油菜籽中B arnase基因 的实时荧光定量PCR检测 |
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| 引用本文： | 潘良文,田风华,张舒亚. 转基因抗草丁膦油菜籽中B arnase基因 的实时荧光定量PCR检测[J]. 中国油料作物学报, 2006, 28(2): 194-198 |
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| 作者姓名： | 潘良文 田风华 张舒亚 |
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| 作者单位： | 1. 上海出入境检验检疫局,上海200135; 2. 华东理工大学生物工程学院,上海200237
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| 基金项目： | 国家标准专项上海地方实施项目;上海市科委资助项目 |
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| 摘 要： | 利用荧光定量PCR技术,对进口抗草丁膦油菜籽中的B arnase基因进行了定量检测,分别建立了抗草丁 膦油菜籽参照样品外源B arnase基因和内标准HMG基因Ct值与模板量之间的标准曲线和线性回归方程,运用所 建立起来的方法对14批油菜籽样品进行了定量分析。 转基因油菜籽; 草丁膦; B arnase基因; HMG基因; 定量PCR
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| 关 键 词： | 转基因油菜籽 草丁膦 Barnase基因 HMG基因 定量PCR |
| 文章编号： | 1007-9084（2006）02-0194-05 |
| 收稿时间： | 2005-09-12 |
| 修稿时间： | 2005-09-12 |
Real-time quantitative PCR detection of the Barnase gene in genetically modified phosphinothricin-tolerant canola |
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| PAN Liang-wen,TIAN Feng-hua,ZHANG Shu-ya. Real-time quantitative PCR detection of the Barnase gene in genetically modified phosphinothricin-tolerant canola[J]. Chinese Journal of Oil Crop Sciences, 2006, 28(2): 194-198 |
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| Authors: | PAN Liang-wen TIAN Feng-hua ZHANG Shu-ya |
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| Abstract: | The Barnase gene in genetically modified phosphinothricin-tolerant canola was quantitatively detected by rea1-time fluorescent quantitative PCR.The endogenous HMG gene and exogenous Barnase gene were amplified respectively.The standard curve of Ct value amplified by Barnase gene or HMG gene vs amounts of the DNA template was generated and a linear regression equation was obtained,consequently.14 lots of samples from imported rapeseeds were quantitively analyzed by the established method. |
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| Keywords: | Genetically modified canola Phosphinothricin Barnase gene HMG gene Real-time quantitative PCR |
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