Somatic embryogenesis from immature and mature zygotic embryos ofCryptomeria japonica I: Embryogenic cell induction and its morphological characteristics |
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| Authors: | Shinjiro Ogita Hisako Ishikawa Takafumi Kubo Hamako Sasamoto |
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| Affiliation: | (1) Cell Manipulation Laboratory, Bio-resources Technology Division, Forestry and Forest Products Research Institute, 305-8687 Ibaraki, Japan;(2) Department of Environmental and Natural Resource Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 183-8509 Tokyo, Japan |
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| Abstract: | Embryogenic cells (ECs) of sugi (Cryptomeria japonica D. Don) were induced from immature and mature zygotic embryos cultured on different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D)-containing modified Campbell and Durzan medium. The rate of induction of ECs varied depending on the stage of embryos collected. The highest percentage of induction (35%) was obtained with immature zygotic embryos collected July 18 and July 30, 1997, when 1  M of 2,4-D was added to the induction medium. The ECs easily proliferated when subcultured in a medium of the same composition as the induction medium within 3 weeks. Morphological characteristics of nonembryogenic cells and embryogenic cells of different developmental stages were studied under an inverted fluorescence microscope.Part of this work was presented at the 109th annual Japanese Forestry Society meeting at Tochigi, April 1998 |
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| Keywords: | Cryptomeria japonica Zygotic embryo Embryogenic cell Somatic embryogenesis |
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