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Genetic relationship of Mediterranean mandarins (Citrus deliciosa Tenore) using RAPD markers
Authors:M A Machado  H D Coletta Filho  M L P N Targon  J Pompeu Jr
Institution:(1) Centro de Citricultura Sylvio Moreira/Instituto Agronômico de Campinas, C.P. 04-CEP 13490-970 Cordeirópolis, SP, Brazil
Abstract:Summary Random amplified polymorphic DNA (RAPD) analysis was carried out to evaluate polymorphism and genetic similarity between 39 Mediterranean mandarin genotypes. One hundred eleven amplification products were identified using 21 random primers. An average of 2.2 RAPD markers was obtained for each primer, corresponding to 42% of the amplification products. Genotype-specific RAPD markers were also found, mainly in known hybrids. UPGMA cluster analysis revealed the low level of genetic variation between accessions of Mediterranean mandarins, whereas their hybrids with other Citrus species showed greater genetic dissimilarity. Twenty accessions yielded very similar patterns, suggesting either that they could be a single clone, or that the technique was not able to detect genomic variation. However, for the other specimens genetic polymorphism can easily be detected by RAPD, although the genetic variation between accessions was quite low. The large number of hybrids and the low polymorphism between accessions support the hypothesis that Mediterranean mandarins are all true hybrid of Common mandarins (Citrus reticulata Blanco).
Keywords:Citrus deliciosa  Mediterranean mandarins  germplasm analysis  RAPD markers
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