文蛤CDK1基因在选育与自然群体早期生长阶段中的表达特征

为探究细胞周期素依赖性激酶1（CDK1）在选育群体红文蛤和自然群体黄文蛤（）的cDNA序列，该基因cDNA序列全长为1623 bp，其中包括353 bp的5''末端非翻译区（UTR），370 bp的3''UTR，900 bp的开放阅读框（ORF），共编码299个氨基酸。预测氨基酸序列具有较高的保守性，包含STKc-CDK1-euk结构域、丝氨酸/苏氨酸蛋白激酶家族特征序列DLKPQN和G/V-T/S-X-X-Y/F-X-A-P-E、与cyclin B结合相关的保守序列PSTAIRE，以及与ATP相结合的保守序列GXGXXG和K33。组织表达结果显示，基因在各个组织中广泛表达，其中性腺中的表达量最高，其次是外套膜。不同条件（光照、饵料）培育实验中，光照强度分别为2.16~778 Lux和1.48~70.6 Lux，饵料密度分别为20×10⁴ ind/mL和5×10⁴ ind/mL，结果显示，红文蛤苗种表现出比黄文蛤苗种更快的生长速度；各养殖条件下<0.05）。由此推测，基因在文蛤苗种生长中发挥重要作用，且选育后的红文蛤较黄文蛤拥有更好的生长性能。本实验将为进一步研究文蛤生长发育相关机制提供参考，也为文蛤新品种选育奠定理论基础。

Expression analysis of the CDK1 gene between selected and natural populations of juvenile Meretrix meretrix

Cyclin-dependent kinases (CDKs) are members of the serine/threonine protein kinase family and key regulators of the cell cycle transitions in eucaryon. CDK1, also known as Cdc2 or p34cdc2, is the first documented CDK and is vital for mitosis and meiosis. CDK1 functions as a catalytic subunit by binding with its regulatory subunit cyclin B to form a complex at the transition from G2 to M phase. The gene has been described in several species, but investigations in mollusks are lacking. To study the function of the gene in juveniles of selected (red clams) and natural (yellow clams) populations of gene via rapid amplification of cDNA ends (RACE). The cDNA is 1623 bp long and contains a 353 bp 5'' untranslated region (UTR), a 370 bp 3'' UTR, and a 900 bp open reading frame (ORF) encoding 299 amino acids. The predicted amino acid sequence of exhibited serval conserved characteristics, including a STKc-CDK1-euk domain, specific sequences of the CDK family DLKPQN and G/V-T/S-X-X-Y/F-X-A-P-E, the cyclin B binding site PSTAIRE, and ATP-coupled-sites GXGXXG and K33. Sequence alignments revealed that kinases from other species. The tissue distribution analyses showed that is expressed in all of the tested tissues. The highest expression was in the gonad, followed by the mantle, suggesting that plays an important role in the cells with active division in the gonad and mantle. In different aquaculture conditions experiments, the red clams grew faster than the yellow clams under high and low light and under high and low algal concentrations. The expression profiles of under different aquaculture conditions were consistent with the growth results that expression level was also higher in the red clams. Therefore, we speculate that is closely related to early juvenile growth and that the light and algal concentrations also play important roles (inappropriate conditions may inhibit juvenile growth). The red clams may possess better growth and development characteristics than the yellow clams. The results of this study contribute to a better understanding of the mechanisms of growth and provide basic information for breeding new varieties of .