四君子汤对脾虚北京鸭胃肠组织中血管活性肠肽和生长抑素mRNA表达的影响

为从分子水平阐明脾虚与胃肠激素之间的关系以及健脾中药四君子汤防治脾虚的机制，首次用北京鸭复制脾虚证模型，以半定量的RT—PCR方法检测其腺胃、十二指肠和空肠血管活性肠肽（VIP）和生长抑素（SS）mR—NA表达的变化。结果表明，脾虚组腺胃VIPmRNA表达显著高于对照组（P〈0．05），十二指肠和空肠VIPmR—NA表达有所升高（P〉0．05）；脾虚组腺胃（P〈0．01）、十二指肠（P〈0．05）和空肠（P〈0．01）SSmRNA表达显著低于对照组。经四君子汤预防和治疗后二者恢复到接近对照组水平。提示脾虚证与VIP和SS有一定关系，四君子汤对VIP和SS水平的影响可能是该方治疗脾虚证的作用机制之一。

Effect of Sijunzi Decoction on the Expression of mRNA of VIP and SS of Beijing Duck with Spleen Deficiency

The object of this paper is to explore the mechanism of Sijunzi decoction on spleen deficiency(SD)models and the relationship between spleen deficiency syndrome and gastrointestinal hormones.Spleen deficiency model was established in Beijing ducks by injected Reserpine,the expression of mRNA of VIP and SS gene in proventriculus,duodenum and jejunum were measured by semi-quantitative RT-PCR.Results indicated that the expression of mRNA of VIP gene in proventriculus of SD ducks were significantly higher(P<0.05) than that of control group while the expression of mRNA of SS gene were lower(P<0.01 in proventriculus;P<0.05 in duodenum;P<0.01 in jejunum) than that of the control group. After treated with Sijunzi decoction,the expression of VIP and SS gene of SD duck were closed to that of control.The data showed that spleen deficiency syndrome was closely related with the change of gastrointestinal hormones(VIP and SS).The regulation effect of Sijunzi decoction on VIP and SS might be one of the therapeutic mechanisms in curing spleen deficiency syndrome.