三氮苯类除草剂对水稻染色体结构、蛋白质含量及组分的影响

比较研究了不同浓度三氮苯类除草剂对水稻幼苗生长、叶绿素及可溶性蛋白质含量、根尖分生组织染色体结构、分生组织和叶绿体蛋白质组分的影响。结果表明，在0.1 mg /L Atrazine处理水稻后培养1周。幼苗高度由(10.8±1.7) cm（对照）降至 (8.2±0.7) cm，叶绿素含量由(1.07±0.013) mg/g FW（对照）降至(0.97±0.013) mg/g FW,

The Effect of Triazine Herbicide Atrazine on the Chromosome Structure, Protein Content and Compositions in Oryza sativa L.

In this paper, the comparative studies on the effects of triazine herbicide Atrazine on seedlings growth, contents of chlorophyll and soluble protein, chromosome structure of root meristem, protein compositions of root meristems and chloroplast have been made in Oryza sativa. The seedlings growth was obviously reduced from (10.8±1.7) cm (control) to (8.2± 0.7) cm, chlorophyll contents were decreased from (1.07±0.013) mg/g FW(control) to (0.97±0.013) mg/g FW, and soluble protein contents were decreased from 40.4 mg/g FW (control) to 29.3 mg/g FW when seedlings were treated with 0.1 mg/L Atrazine for 7 days. 0.68% cells with chromosome condensation and 0.86 % cells with micronuclei were revealed in root meristems when the root meristems were treated with 0.01 mg/L Atrazine. The frequency of chromosome condensation and micronuclei formation were increased with the increase of Atrazine concentration from 0.001 mg/L to 10 mg/L(Table 4). In 2D SDS-PAGE, 2 new protein spots were induced and 8 protein spots were lost in root meristems, 1 protein spot was lost and contents of 3 protein spots were decreased in the chloroplast when seedlings were treated with 0.1 mg/L Atrazine. The threshold concentration obviously affected seedling growth, contents of chlorophyll and soluble protein, protein compositions of root meristems and chloroplast was 0.1 mg/L. The threshold concentration induced the chromosome condensation and micronuclei formation was 0 .001 mg/L.