| 物理与化学诱变筛选产石杉碱甲高产菌株的研究 |
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| 引用本文： | 周树良,温拥军,徐宁,龚晓,洪亚辉. 物理与化学诱变筛选产石杉碱甲高产菌株的研究[J]. 湖南农业科学, 2009, 0(10): 1-3,8 |
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| 作者姓名： | 周树良 温拥军 徐宁 龚晓 洪亚辉 |
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| 作者单位： | 1. 湖南农业大学生物科学技术学院,湖南,长沙,410128
2. 湖南化工职业技术学院,湖南,株洲,412004 |
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| 摘 要： | 以千层塔内生真菌SHB为出发菌株，采用微波、紫外线、Co60射线、亚硝酸和硫酸二乙酯对内生真菌孢子进行诱变。诱变孢子悬液通过在PDA平板继代培养2次，把单菌落孢子转接至PD培养液中进行发酵培养，利用高效液相色谱检测发酵液，筛选出一株遗传稳定，产石杉碱甲能力较高的高产菌株wB3。其发酵产物中石杉碱甲为原始菌株的3．04倍。
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| 关 键 词： | 内生真菌 诱变 石杉碱甲 |
Selection of Huperzine A High-producing Strain by Physical and Chemical Mutation |
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| ZHOU Shu-liang,WEN Yong-jun,XU Ning,GONG Xiao,HONG Ya-hui. Selection of Huperzine A High-producing Strain by Physical and Chemical Mutation[J]. Hunan Agricultural Sciences, 2009, 0(10): 1-3,8 |
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| Authors: | ZHOU Shu-liang WEN Yong-jun XU Ning GONG Xiao HONG Ya-hui |
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| Affiliation: | ZHOU Shu-liang1,WEN Yong-jun2,XU Ning1,GONG Xiao1,HONG Ya-hui1 (1.College of Bioscience and Biotechnology,HNAU,Changsha 410128,PRC,2. Hunan Chemical Industry Vocational Technology College,Zhuzhou 412004,PRC) |
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| Abstract: | Abtract: The spores of endophytic fungi SHB of Hupezia serrata were mutated by microwave, ultraviolet ray, Co60 ray, nitrous acid and diethyl sulfate. Then, the suspension of mutated spores was sub-cultured twice in PDA plate, and the single colony spores were transplanted to PD medium for ermentation. Finally, a Huperzine A high-producing strain - wB3 with stable inheritance was selected by high erformance liquid chromatography (HPLC). The content of Huperzine A in fermenting product of wB3 is 3.04 times more than the primitive fungi SHB's. |
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| Keywords: | endophytie fungi mutation huperzine A |
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