| 胡桐愈伤组织诱导条件初报 |
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| 引用本文： | 邵志芳,罗焕亮,郭勇.胡桐愈伤组织诱导条件初报[J].林业科技,2006,31(4):1-3. |
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| 作者姓名： | 邵志芳 罗焕亮 郭勇 |
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| 作者单位： | 深圳市莲花山公园,广东,深圳,518049;深圳出入境检验检疫局,广东,深圳,518045;华南理工大学生物工程学院,广东,广州,510641 |
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| 基金项目： | 广州市科技新星计划资助项目（2000-K-003-01） |
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| 摘 要： | 为了通过植物细胞培养获取胡桐抗HIV的活性物质,首次报道胡桐愈伤组织的诱导条件。研究结果表明:胡桐幼苗的叶、茎段与胚根均可诱导出愈伤组织,其中以茎段的诱导率最高,达95.6%,而胚乳难以诱导愈伤组织。胡桐愈伤组织生长与培养基中无机盐含量有关,在B5、MS、White三种不同的基本培养基中,以B5最适于胡桐愈伤组织的诱导,MS次之,White最差。
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| 关 键 词： | 胡桐 愈伤组织 外植体 诱导 |
| 文章编号： | 1001-9499（2006）04-0001-03 |
| 收稿时间： | 2006-02-27 |
| 修稿时间： | 2006年2月27日 |
A Preliminary Report on Callus Induction of Calophyllum inophyllum |
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| SHAO Zhifang.A Preliminary Report on Callus Induction of Calophyllum inophyllum[J].Forestry Science & Technology,2006,31(4):1-3. |
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| Authors: | SHAO Zhifang |
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| Institution: | Shenzhen Lianhua Mountain Garden, Guangdong 518049 |
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| Abstract: | In order to obtain the anti-HIV substance in Calophyllum inophyllum by plant cell culture,the calli-induced factors of C.inophyllum were firstly reported.The calli could be induced from young leaves,stem and radicles of C.inophyllum seedling on MS medium but could not from endosperms.Of the explants above,the highest calli-inducing frequency with 95.6% was observed from stem.The calli growth was in relation to the concentration of inorganic salts in the medium.The best basic medium for the calli growth was B_5 following with MS and White. |
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| Keywords: | Calophyllum inophyllum Callus Explant Induction |
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