Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler) |
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Authors: | Wallowitz Mikhael Peterson W Rich Uratsu Sandie Comstock Sarah S Dandekar Abhaya M Teuber Suzanne S |
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Institution: | Department of Internal Medicine, Division of Rheumatology, Allergy and Clinical Immunology, University of California-Davis School of Medicine, Davis, CA, USA. |
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Abstract: | Allergy to walnut is the most frequently reported tree nut allergy in the United States. Walnut 2S albumin, a vicilin-like protein, and a lipid transfer protein allergen have previously been described. Our objective was to clone and express a cDNA encoding a legumin group protein, assess IgE-binding with sera from walnut allergic patients, and investigate cross-reactivity with selected nuts. Primers were used to obtain the cDNA by 5' and 3' rapid amplification of cDNA ends from walnut mRNA. The cDNA was subcloned into the pMAL-c2X vector and the recombinant fusion protein, named rJug r 4, was expressed in Escherichia coli. The obtained cDNA encoded a precursor protein with a predicted molecular weight of 58.1 kD, which showed significant sequence homology to hazelnut and cashew legumin allergens. Serum IgE from 21 of 37 (57%) patients bound the rJug r 4 fusion protein. In vitro cross-reactivity was demonstrated with hazelnut, cashew, and peanut protein extracts. |
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