人工感染鸭东方次睾吸虫病的病理组织学研究

本文应用光镜（LM）、扫描电镜（SEM）和透射电镜（TEM）对人工感染东方次睾吸虫家鸭的肝脏、胆囊进行了病理组织学研究。LM显示:肝实质细胞脂肪变性,胆小管广泛增生,肝间质结缔组织明显增生,嗜酸性细胞浸润。胆囊部分粘膜上皮脱落,粘膜下血管充、出血明显,炎性细胞浸润。SEM显示:胆囊壁部分粘膜脱落而显光滑。TEM显示:肝细胞滑面内质网明显增多,溶酶体和线粒体也有增加现象,溶酶体多集中在毛细胆管附近,部分细胞可见多量脂滴,有些肝细胞破溃,附近有炎性细胞浸润。肝血窦扩张,可见多量红细胞、嗜酸性颗粒白细胞和巨噬细胞。内皮细胞筛板部分增厚,内皮窗孔缩小。枯否氏细胞质内蠕虫样结构增多,吞噬能力加强。肝脏毛细胆管增生,部分可见胆栓位于管腔内,微绒毛变短,周围肝细胞外质膜增厚。

PATHOHISTOLOGICAL STUDY ON OPISTHORCfflASIS OF DUCKS INFECTED WITH METORCHIS ORIENTALIS ARTIFICIALLY

The livers and gallbladders of ducks infected with Metorchis orientals artificially were observed by light microscopc(LM) and scanning electron microscope (SEM) and transmission electron microscopc(TEM). LM showed the fatty degeneration of the hcpatocytcs,proliferation of bile canaliculi and connccivc tissue,and heterophilic infitration in the livers. Part of the mucosal epithelium was detached from the linning of the gallbladders. Hypcrcmia and hemorrhage and inflammatory infitration were also observed. SEM showed that part of mucosal epithelium was detached from the linning of the bile bladder,and smoothness on the surface of thelinning of the gallbladders. TEM showed that the smooth cndoplasmic rcticulum in hcpatocytcs increased, so did the mitochondria and lysosomcs which often concentrated around the bile canaliculi. Fatty droplets appeared in some hcpatocytcs. Some hcpatocytcs became nccrobiosis.ncar by, inflammatory infitration were observed. Enlargement of hepatic sinusoid which contained crythrocytcs,cosinophils,macrophagcs was observed. Worm-like srtucturc in Kupffcr's cells was increased,and phagocytosis grew. The bile canaliculus in the liver proliferated, and the bile cmbolus were seen in some bile canaliculi in which microvilli became shorter, the cx-traplasmic membrane of the hcpatocytcs became thicker.