油菜素甾醇对沟叶结缕草长期培养愈伤组织生长和再生的影响

为了维持长期培养植物愈伤组组织的高再生率,以应用于体细胞无性系进行变异筛选.本研究以继代15年的沟叶结缕草[Zoysia matrella(L.)Merr.]胚性愈伤组织为材料,用不同浓度的表油菜素内酯(EBL)处理,研究油菜素甾醇(BR)对其生长和再生的影响,选出可提高长期离体培养愈伤组织生长及再生效率的最优EBL浓...

Effect of Brassinosteroid on Long-Term-Maintained Callus Growth and Regeneration of Zoysia Matrella[L.]Merr.

In order to maintain a high regeneration rate of plant callus with a long time,and to identify somaclnal variation,in this study,the embryonic calli of Zoysia matrella subcultured for 15 years were treated with different concentrations of epibrassinolide(EBL).The effects of BR the its growth and regeneration of long-term in vitro cultured callus were studied,and the optimal EBL concentration was selected to improve the growth and regeneration efficiency,which provided a reference for optimizing the subculture and regeneration system of Z.matrella.Meanwhile,to investigate the mechanism of the antioxidant system during embryogenesis of Z.matrella,catalase(CAT),peroxidase(POD),superoxide dismutase(SOD)activities,and malondialdehyde(MDA)content in callus and regenerated plants treated with EBL were examined.The results showed that 0.02μmol·L^-1EBL had the most significant promotion for callus growth and regeneration.The embryogenesis of callus was best maintained under the treatment of 0.02μmol·L^-1EBL,the diameter increased by 131.11%while the fresh weight increased by 43.8%.High concentrations of EBL(>0.10μmol·L^-1)inhibited the embryogenic maintenance and regeneration of callus.The antioxidant reaction system in plants was activated by EBL treatment.The activity of POD decreased during subculture;the activities of CAT,POD,and SOD were improved during the regeneration.Additionally,the content of MDA decreased noticeably during the subculture and regeneration,which suggests that BR may regulate the physiological and biochemical processes during somatic embryogenesis by activating the antioxidant response and strengthening the antioxidant reaction in the process of subculture and regeneration.This study provides a solution to alleriated the problem that the regeneration rate of callus in long term culture decreases with the growth of culture time.