冷激结合水杨酸处理对黄瓜果实冷害及能量和脯氨酸代谢的影响

为探讨冷激结合水杨酸处理减轻黄瓜果实冷害的作用及其机理,本试验分别研究了冷激(0℃冰水混合物)、水杨酸(1 mmol·L-1)以及二者结合(含有1 mmol·L-1水杨酸的0℃冰水混合物)3种处理方式对黄瓜果实低温贮藏期间能量和脯氨酸代谢的影响.结果表明,冷激、水杨酸以及二者结合处理均能抑制黄瓜果实冷害程度、相对电导率...

Effects of Cold Shock Combined With Salicylic Acid Treatment on Chilling Injury,Energy and Proline Metabolism of Postharvest Cucumber Fruit

To investigate the mechanism of cold shock combined with salicylic acid treatment on chilling injury of cucumber fruit,the effects of cold shock(0℃ice-water),salicylic acid(1 mmol·L^-1)and the combination of cold shock and salicylic acid(0℃ice-water containing 1 mmol·L^-1salicylic acid)treatments on energy and proline metabolism in cucumber fruit stored at low temperature were studied,respectively.The results indicated that the treatments of cold shock,salicylic acid and cold shock combined with salicylic acid effectively alleviated the chilling injury,and inhibited the relative conductivity and the increase of malondialdehyde(MDA)content in cucumber fruit.In addition,these three treatments markedly increase the activities of Ca²⁺-ATPase,H⁺-ATPase,succinate dehydrogenase(SDH)and cytochrome C oxidase(CCO),retarded the decline of adenosine triphosphate(ATP)and adenosine diphosphate(ADP)content,inhibited the increase of adenosine monophosphate(AMP)content,and maintained higher energy charge compared with the control.The results also demonstrated that three treatments improvedΔ¹-pyrroline-5-carboxylate synthetase(P5 CS)and ornithine-δ-aminotransferase(OAT)activities and inhibited proline dehydrogenase(PDH)activity,which promoted the accumulation of proline of cucumber fruit.And among these three treatments,the effiency of cold shock combined with salicylic acid treatment was the best.This study provides theoretical basis for alleviation of cucumber fruits.