利用ISSR和SSR标记分析芒荻类植物资源遗传多样性

利用ISSR和荧光SSR技术对22种芒荻类植物的遗传多样性进行分析,筛选出具有多态性的ISSR和SSR引物各12对,其中ISSR引物共扩增出216个多态性位点,多态性比率为96.86%,Neis指数为0.301 8,平均Shannon指数为0.462 8;SSR引物共扩增出111个多态性位点,多态性比率为67.68%,Neis指数为0.111 5,平均Shannon指数为0.193 3。UPGMA聚类分析结果表明,22份芒荻类植物资源中除五节芒外,相同地域内的芒、荻和南荻在遗传学上难以区分;芒荻类植物资源的遗传分化与其种源的地域分布有一定相关性。Mantel检验显示,2种分子标记在22份芒荻类植物资源间相关不显著(r=0.374,P0.05)。

Genetic diversity of Miscanthus & Triarrhena based on ISSR and SSR analysis

The genetic differences among 22 germplasm resource were analyzed useing fluorescent polymorphic simple sequence repeats(SSRs)and inter-simple sequence repeats(ISSRs)to identify their genetic diversity.Polymorphic ISSR and SSR primers were screened,each with 12 pairs.The results showed that 12 ISSR primer pairs produced 216 polymorphic bands(96.86%),Nei''s gene heterozygosity was 0.301 8,Shannon index was 0.462 8;12 SSR primer pairs produced 111 polymorphic loci(67.68%),Nei''s gene heterozygosity was 0.111 5,Shannon index was 0.193 3.UPGMA cluster analysis indicated that 22 germplasm resource,in addition to Miscanthus floridulus,the Miscanthus sinensis,Triarrherca sacchariflora and Triarrhena lutarioriparia in the same geographic area can not be effectively identified in genetics;there were some correlations between Miscanthus & Triarrhena and geographic distribution.Mantel test revealed that the correlation of genetic similarity matrix between ISSRs and SSRs was not significant(r=0.374,P > 0.05)for 22 species.