牛磺酸对断奶小鼠小肠发育的影响

为研究牛磺酸对断奶小鼠小肠结构和功能的影响,采用单因子设计,48只体重为(12±2)g的昆明小鼠随机分为6组,每组8只,雌雄各半,分为对照组和试验组,对照组饮用普通自来水,试验组在饮水中添加2.5、5.0、10.0、20.0和40.0g/L牛磺酸,试验期31d。观察牛磺酸对小肠和肝脏重量,空肠绒毛形态、细胞凋亡、小肠核酸和蛋白质含量,以及血清指标的影响。结果表明:1)饮水中添加牛磺酸可显著提高小鼠小肠绝对重量和相对重量(P0.05),且呈现二次曲线关系(PQ0.05);可线性提高小肠绝对长度、肝脏绝对重量和相对重量(PL0.05)。2)2.5、5.0和10.0g/L组与对照组相比,显著降低空肠肠腺(肠隐窝)深度(P0.05);回肠Prot/DNA随牛磺酸添加量提高而线性提高(PL0.05)。3)与对照组比较,5.0g/L添加组的细胞凋亡指数显著降低(P0.05),但40g/L添加组有升高的趋势。4)随着牛磺酸添加量的提高,回肠MDA含量有降低的趋势(P=0.07),空肠GSHPx呈现先升高再降低的二次曲线关系(PQ0.05),但不影响血清IGF-I水平。研究结果表明,饮水添加中低浓度的牛磺酸可提高小鼠小肠长度和重量,降低肠隐窝深度,减少细胞凋亡。

Effects of taurine on the intestinal development of mice

Taurine has many biological roles for animals.The experiment was conducted to determine the effect of dietary taurine on the intestinal development of mice.A single factor design was adopted,and a total of 48 KM mice were randomly allocated into 6 groups with 4 males and 4 females in each group.The tap water was supplemented with 2.5,5.0,10.0,20.0 and 40.0 g/L taurine,respectively.The commercial diets were fed ad libitum.The mice were raised for 31 days to observe the effect of taurine on the gross growth of small intestine and liver,morphology,cell apoptosis and antioxidant capacity.The results showed as follows:1)The weight and the relative weight of small intestine were significantly increased (P<0.05).Besides,the length of small intestine and the weight of liver increased linearly (P_L<0.05).2)Taurine supplemented with 2.5,5.0 and 10.0 g/L also decreased the crypt depth in the jejunum (P<0.05).In addition,Prot/DNA in ileum also increased linearly (P_L<0.05).3)Compared with the control group,taurine supplemented with 5.0 g/L could decrease the apoptosis index,while higher levels (40.0 g/L) increased it.4) MDA contents in ileum decreased (P=0.07) and GSH-Px in the jejunum was related with the dietary taurine supplement level quadraticly,taurine had no influence on serum IGF-I levels.It can be concluded that taurine in small dosage exerts beneficial effects for development of the small intestine of mice,decreasing the crypt depth and mucosal apoptosis.