| 基于LC-ESI-MS/MS技术与生物信息学的蚕豆种子贮藏蛋白亚基鉴定 |
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| 引用本文： | 刘玉皎,侯万伟. 基于LC-ESI-MS/MS技术与生物信息学的蚕豆种子贮藏蛋白亚基鉴定[J]. 中国农业大学学报, 2014, 19(1): 37-42 |
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| 作者姓名： | 刘玉皎 侯万伟 |
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| 作者单位： | 青海省农林科学院 青海大学农林科学院/青海省高原作物种质资源创新与改良利用国家重点实验室培育基地, 西宁 810016;青海省农林科学院 青海大学农林科学院/青海省高原作物种质资源创新与改良利用国家重点实验室培育基地, 西宁 810016 |
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| 基金项目： | 国家973计划前期专项（2010CB134408）；现代农业产业技术体系建设专项（CARS-09）；青海省高校“135”高层次人才培养工程 |
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| 摘 要： | 蚕豆蛋白亚基是蚕豆种子贮藏蛋白重要组成部分,深入鉴定蚕豆蛋白亚基,对了解蚕豆种子蛋白不同亚基的结构和功能具有重要意义。本研究应用液相色谱、电喷雾离子化与串联质谱联用技术和生物信息技术对蚕豆种子贮藏蛋白6个特异亚基进行了质谱鉴定。结果表明:这些特异亚基中的64、47、42及38ku分别鉴定出4个蛋白:豌豆球蛋白、豆球蛋白前体、假定糖结合蛋白、LEGB7_VICFA;97ku亚基蛋白为分子伴侣GroEL;96ku亚基蛋白由6个具有不同代谢功能的蛋白组成。
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| 关 键 词： | 蚕豆 蛋白亚基 串联质谱 鉴定 |
| 收稿时间： | 2013-05-20 |
Identification for seed store protein subunits of faba bean based on LC-ESI-MS/MS and bio-informatics |
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| LIU Yu-jiao and HOU Wan-wei. Identification for seed store protein subunits of faba bean based on LC-ESI-MS/MS and bio-informatics[J]. Journal of China Agricultural University, 2014, 19(1): 37-42 |
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| Authors: | LIU Yu-jiao and HOU Wan-wei |
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| Affiliation: | Qinghai University Academy of Agricultural and Forestry Science/Cultivating Base of National Key Lab-Plateau Crop Gene Innovatedand Utilized in Qinghai Province, Qinghai Academy of Agricultural and Forestry Science, Xining 810016, China;Qinghai University Academy of Agricultural and Forestry Science/Cultivating Base of National Key Lab-Plateau Crop Gene Innovatedand Utilized in Qinghai Province, Qinghai Academy of Agricultural and Forestry Science, Xining 810016, China |
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| Abstract: | The protein subunits are important components for store protein in faba bean seed,since it is essentially the first step to further understand their structures and functions of these protein subunits in detail.Six specific protein subunits of faba bean were identified by using liquid chromatograph (LC),LC-ESI-MS/MS and bio-information approaches in this study.The results indicated that four proteins were identified from each of the 64,47,42 and 38 ku specific protein subunits.The four proteins were convicilin,legumin a precursor,putative sucrose binding protein and LEGB7_VICFA;97 ku encoded the same protein as GroEL;96 ku was composed of six proteins with different metabolic characteristics. |
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| Keywords: | faba bean protein subunit MS/MS identification |
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