雌二醇对大鼠下丘脑神经激肽B表达的影响

为研究雌二醇(Estradiol,E2)对大鼠下丘脑神经激肽B(Neurokinin B,NKB)表达的影响,采用免疫组化PV-9003二步法以及DAB显色技术,对外源性E2处理组(E2组)、花生油处理组(C组)、卵巢摘除+E2组(OVX+E2组)和卵巢摘除组(OVX组)大鼠下丘脑的NKB进行检测。结果表明:大鼠阴门开启时间E2组(29.00±0.707)与OVX+E2组(30.20±0.084)早于对照组(43.60±2.074)(P0.01)。NKB主要分布于所有大鼠下丘脑的弓状核、腹内侧核和室旁核,且OVX组和C组室周核上也有少量表达(P0.05),但各组间NKB的平均光密度值有差异,OVX组大鼠下丘脑弓状核(0.33±0.49)、腹内侧核(0.31±0.54)和室旁核(0.30±0.55)均显著高于OVX+E2、C和E2组(P0.05)。雌激素抑制大鼠下丘脑NKB的表达,NKB可能通过雌激素调节生殖作用。

Effect of estrogen on the expression of neurokinin B in rat hypothalamus

In order to study the effect of estrogens on the expression of neurokinin B in rat hypothalamus,the immunohistochemical PV-9003 2-step method and DAB staining technology were used to investigate the expression of NKB in the hypothalamus of rats in different groups,that is,the rats were treated by exogenous estradiol (Group E₂),peanut oil (Group C),estradiol after ovariectomy (Group OVX+E₂) and ovariectomy (Group OVX) respectively.The results showed that the day of vaginal opening in the rats of group E₂ (29.00±0.707) and group OVX+ E₂ (30.20±0.084) was more significantly earlier than that of the control group (43.60±2.074) (P<0.01).NKB were mainly distributed in the arcuate nucleus,ventromedial nucleus and paraventricular nucleus and a little ones in the periventricularis nucleus in the rats of the group OVX and group C (P<0.05).However,the mean optical density value of NKB in the different groups was different,and the data in the arcuate nucleus (0.33±0.49),ventromedial nucleus (0.31±0.54) and paraventricular nucleus (0.30±0.55) of OVX rats were significantly higher than that in the group OVX+E₂, group C and group E₂.It showed that the estrogen inhibited the expression of NKB in hypothalamus of rats,and NKB may play a role in the regulation of reproductive function by estrogen.