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| 乙肝表面抗原基因表达载体的构建及对百脉根的转化 | |
| 引用本文： | 贺红霞,林春晶,王铭,董英山. 乙肝表面抗原基因表达载体的构建及对百脉根的转化[J]. 农业生物技术学报, 2007, 15(1): 115-118 |
| 作者姓名： | 贺红霞 林春晶 王铭 董英山 |
| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,公主岭,136100;吉林农业大学,长春,130118 2. 吉林省农业科学院生物技术研究中心,公主岭,136100 3. 吉林农业大学,长春,130118 |
| 基金项目： | 科技部专项转基因杂交大豆育种研究及产业化项目 |
| 摘 要： | 利用PCR成功的从乙肝患者血清中扩增出乙肝表面抗原基因,并构建了含有此基因的植物表达载体pBHB。用根癌农杆菌(Agrobacterium tumefaciens)介导叶盘法将乙肝表面抗原基因转入百脉根(Lotus corniculatus),从转化植株中提取DNA做PCR检测、Southern杂交,阳性结果验证了目的基因已整合到百脉根的基因组中,转化效率为20%,证实转化的载体真实高效。 |
| 关 键 词： | 乙肝表面抗原基因 载体构建 百脉根 转化 |
| 文章编号： | 1006-1304（2007）01-0115-04 |
| 收稿时间： | 2006-03-28 |
| 修稿时间： | 2006-06-18 |
Expression Vectorion Construction of Hepatitis B Surface antigen (HBsAg) Gene and Transformation to Crowtoe(Lotus corniculatus) |
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| HE Hong-xia,LIN Chun-jing,WANG Ming,DONG Ying-shan. Expression Vectorion Construction of Hepatitis B Surface antigen (HBsAg) Gene and Transformation to Crowtoe(Lotus corniculatus)[J]. Journal of Agricultural Biotechnology, 2007, 15(1): 115-118 | |
| Authors: | HE Hong-xia LIN Chun-jing WANG Ming DONG Ying-shan |
| Affiliation: | 1. Center of Agribiotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China; 2.Jilin Agriculture University, Changchun 130118, China |
| Abstract: | To construct the high effect expression plasmid, the Hepatitis B surface antigen (HBsAg) gene was cloned from Hepatitis B virus DNA by using PCR method, and it was inserted into vector of pBin438 .The plant expression vector pBHB was obtained. Crowtoes(Lotus corniculatus)transformed by Agrobacterium-mediated method were analyzed for the integration of the transgene by PCR and Southern blot. The positive band indicated that the exterior gene had been integrated into crowtoe. There were 20% positive plants, which showed the expression vector pBHB was high effective. |
| Keywords: | HBsAg vector construction crowtoe transformation |
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