基于SLAF-seq技术开发长穗偃麦草染色体特异分子标记

长穗偃麦草1E及7E染色体上带有重要的抗赤霉病基因, 开发大量相关染色体特异分子标记有助于准确定位抗性基因及获得可用于辅助育种紧密连锁的标记。基于SLAF-seq技术, 获得了368个长穗偃麦草1E染色体特异片段, 随机选取80个特异片段设计引物, 开发了20个长穗偃麦草1E染色体特异分子标记、2个长穗偃麦草基因组特异分子标记及26个其他特异分子标记, 效率达60%。用这些特异标记能稳定检测出不同小麦–长穗偃麦草衍生材料中的1E染色体或片段。通过标记与优良性状的共分离特性, 获得与相关基因紧密连锁的标记, 将为小麦抗性育种中的分子标记辅助选择提供依据。

Development of Specific Molecular Markers for Thinopyrum elongatum Chromosome Using SLAF-seq Technique

The 1E and 7E chromosomes of Thinopyrum elongatum carry important resistance genes to wheat Fusarium head blight. Mapping and utilization of these resistance genes require numerous molecular markers specific to the 1E or 7E chromosome. In this study, we developed 368 specific fragments of Thinopyrum elongatum 1E chromosome using SLAF-seq technique, and randomly selected 80 fragments to design specific primers. Finally, 20 1E-specific, 2 genome-specific, and 26 other specific molecular markers were obtained, with the efficiency up to 60%. All the newly developed markers could amplify the specific bands in different lines derived from wheat–Th. elongatum progenies. According to the cosegregation of the specific markers and elite traits, some markers detected could be closely linked to the genes corresponding to target traits.