桃黄化病植原体昆虫介体的验证及其带毒部位PCR检测

 利用植原体16S rDNA通用引物对采集的北京和天津黄化病桃树总DNA进行巢式PCR检测,证明发病样本的病原为桃黄化病植原体。经过检测昆虫总DNA和经取食过的人工培养液DNA中桃黄化病植原体的16S rDNA,结果表明桃黄化病植原体的有效传播媒介昆虫为桃一点叶蝉。将带毒桃一点叶蝉个体的头部、胸部以及腹部分离,分别在这些部位检测到桃黄化病植原体的16S rDNA,说明桃一点叶蝉的头部、胸部以及腹部都可带毒,表明植原体可从植物汁液进入叶蝉的口针、食道和肠道。

Confirmation of the insect vector transmitting Phytoplasma sp.associated with Peach yellow disease and detection of the Phytoplasma sp.in vector

Phytoplasmas are transmitted by phloem-feeding insects. With universal primers for phytoplasma, the 16S rDNA was amplified by nested-PCR using total DNA extracted from diseased peach samples collected from Beijing and Tianjin district. The results showed that there was phytoplasma associated with the diseased peach samples. Furthermore, the 16S rDNA was amplified for phytoplasma using the total DNA extracted from insects and the artificial medium as the templates. The results confirmed that the leafhopper Erythroneura sudra was the efficient vector for peach yellow phytoplasma. The 16S rDNA was amplified from the cephalosome, thoracic and urosomal regions, respectively. And the result showed that the phytoplasmas were existed in the digestive system of E. sudra. It concluded that phytoplasmas were ingested with plant sap and moved through the stylet’s food canal and the intestinal tract.