基于叶绿体DNA序列的Ceratotropis亚属遗传进化研究

作为Ceratotropis亚属起源中心之一，中国有丰富的Ceratotropis亚属种质资源，但种间遗传进化研究相对落后。为探索该亚属各种间的亲缘关系及遗传演化规律，对Ceratotropis亚属18个种及亚种共110份材料的叶绿体基因组2个内含子(rpl16、rps16)和3个基因(psbA-trnH、rpoB-trnC和trnL-trnF)的间隔区序列进行了研究。结果表明，各序列的信息位点百分率在3.63%~24.28%之间，其中rps16最高，rpoB-trnC最低。序列拼接的Kimura进化距离分析发现，各种间进化距离介于0~0.057之间，V. minima复合体(V. minima、V. riukinensis和V. nakashime)和V. subramaniana的进化距离最大。基于单个序列，利用MEGA5.1和PAUP4.0软件构建的邻接树和最大简约树的结果均将Ceratotropis亚属18个种及亚种划分为2大支，其中第I类包含9个种和亚种，为3个组(Angulares、Ceratotropis和Aconitifoliae)的混合体，第II类也包含9个种和亚种，全部属于Angulares组。系统发育树的分化同质性检验表明其拓扑一致性较高(P=0.87)。psbA-trnH等5个序列的分子系统进化研究对阐明Ceratotropis亚属种间亲缘关系和系统演化具有重要的理论和现实意义。

Genetic Evolution for Vigna Subgenus Ceratotropis Based on Chloroplast DNA Sequences

China has abundant germplasm resources of subgenus Ceratotropis, but the related study of species within this subgenus is much lagged. In order to investigate genetic relationship and evolution process among different species, 110 entries of Ceratotropis were analyzed by using intron rpl16, rps16 and intergenic regions psbA-trnH, rpoB-trnC, and trnL-trnF of chloroplast genome. The results revealed that parsim-info site percentage was from 3.63% to 24.28%, with the highest for rps16 and the lowest for rpoB-trnC. A higher level conservation of sequence was observed within species than inter-species. Kimura-2-parameter distance was between 0 and 0.057 calculated based on five chloroplast assembling sequences. V. minima complex (including V. minima, V. riukinensis and V. nakashime) and V. subramaniana had the longest distance. Phylogenetic trees constructed by neighbor-joining (NJ) and maximum parsimony (MP) methods in MEGA 5.1 and PAUP 4.0 revealed that eighteen species and subspecies were divided into two branches. The group I contained nine species and subspecies grouped into three sections complex, including Angulares, Ceratotropis and Aconitifoliae. The group II contained nine species and subspecies which completely belonged to section Angulares. Partition homogeneity test of phylogenetic trees of the five sequences showed that their topology was highly consistent (P=0.87). The present study provides the important information for clarifying genetic relationship Ceratotropis. among different species within