鹑源减蛋综合征病毒QAVc—94株的酶切图谱分析

选HindⅢ、EcoRⅠ、PstⅠ、SphⅠ、BamHⅠ、BamHⅠ和BglⅠ6种限制酶，对QAVc-94株和AV－127国际标准株的DNA进行酶切图谱比较结果发现，QAVc-94株用上述6种酶酶切得到的片段数分别为10、3、12、4、6、9条，将各片段碱基数相加得出其基因组长34.7kb，略高于国内的报道，而与Zask等的结果相接近，从分子水平上将QAVc-94鉴定为一株EDSV。对照AV－127株的酶切结果与文献报道相符。QAVc-94株酶切图谱分析显示：HindⅢ和EcoRⅠ的酶谱与报道的其他EDSV分离株基本相似，HindⅢ的酶谱与国内研究最多的AA－2长春分离株一致，EcoRⅠ的酶谱结果与Zask等报道的B8／78株相同。而其他4种限制酶的酶切图谱与已报道的各地分离株相比，差异较大，如PstⅠ和BglⅠ多出2－5个识别位点。说明鹌鹑源EDSV已与鸡源、鸭源、鹅源EDSV有较大的变异，是一株血清型相同而基因型不同的鹌鹑减蛋综合征病毒。

Restriction Endonuclease Analysis of Egg Drop Syndrome Viruses Strain QAVc-94

The genomes of QAVc-94 and a foreign standard isolate AV-127 were compared through the analysis of 6 different kinds of restriction endonuclease HindⅢ、EcoRⅠ、PstⅠ、SphⅠ、BamHⅠand BglⅠ.The experimental results corroborated that the DNA of QAVc-94 showed 10,3,12,4,6 and 9 fragments respectively.When the length of every fragment was added,the total genome length is 34.7kb,a little more than the data from other isolates in China,but the same as Zask.L's report.The results of RE analysis on AV-127 were conformed to other reports.RE analysis of QAVc-94 also proved that the Restriction patterns of HindⅢ and EcoRⅠ,was generally similar to that of other EDSV isolates known up to now.The QAVc-94' restriction patterns of HindⅢ was consistent with that of AA-2 EDSV isolate from Changchun which has been discussed widely in China,the QAVc-94's mapping of EcoRⅠ was the same as that of B8/78 isolates reported by Zask.But QAVc-94's restriction patterns and the size of the other four Restriction endonuclease were quite different from other isolates.For example,Bgl Ⅰ and PstⅠ had about 2-5 more recognition sites.Therefore,QAVc-94 was far cry from the EDSVs from chicks and ducks et al.This RE analysis testified that QAVc-94 could be the same serotype,but different genome from the other original EDS-76 viruses.