小鼠卵母细胞氯化锶激活条件

以昆明小鼠为试验对象，研究了氯化锶（SrCl2)浓度，激尖处理时间以及卵龄对卵母细胞SrCl2孤雌活化的影响。hCG注射后18h的小鼠卵母细胞在1.6,5.0,10,20mmol/L SrCl2的激活液中处理10min，各组间的激活率（原核期卵母细胞的比例）无显著性差异（P＞0．05）。10，20mmol/L SrCl2组发育至2－，4－细胞期比率（59．26％，20．37％，63．79％，20．69％）同于1．6，5．0mmol/L 组（20．63％，9．26％，47．06％，7．84）（P＜05）。只有20mmol/L组的少数激活卵母细胞（3．45％）发育至桑椹胚阶段。10，20mmol/L SrCl2组处理20，40，60min，2组间的活化率无显著性差异。处理40min,20mmol/L组的桑椹胚和囊胚发育率（64．44％，22．22％）均显著高于10mmol/L组（30．36％，11．61％），处理60min,10mmol/L组的桑椹胚和囊胚发育率（84．68％，46．77％）均显著高于20mmol/L组（67．46％，28．57％），10mmol/L SrCl2处理，20，40，60，180，360min，其活化率和2－细胞的发育率无显著性差异。60，180min处理组的囊胚发育率（49．58％，47．59％）差异不显著，但2者均显著高于20，40，360min组（4．50％，12．24％和35．53％），后3者组间差异显著。随卵龄的增加，活化率显著增加。hCG注射后16h卵母细胞的活化率（77．78％）显著高于14h的卵母细胞（29．63％），而hCG注射后18h卵母细胞的活化率（49．19％）又显著高于16h的卵母细胞，hCG注射后18，20h卵母细胞的活化率差异不显著（94．19％，85．90％）。hCG注射后14，16，18h卵母细胞激活后的囊胚发育率（6．71％，27．78％，47．67％），各组间差异显著。但hCG注射后20h卵母细胞激活后的囊胚发育率（39．74％）显著低于18h的卵母细胞。

Conditions of oocytes Parthenogenetic Activation with SrCl2 in Mice

In this study the effects of concentration of SrCl 2,duration time of exposure to SrCl 2 and oocyte age on the parthenogenetic activation of mouse oocytes were studied.In the experiment 1,the concentration of SrCl 2 on the parthenogenetic activation of mouse oocytes were studied.Mouse(Kunming) oocytes collected at post hCG injection 18 h were treated in 1 6,5 0,10 and 20 mmol/L SrCl 2 CZB for 10 min respectively,the activation rates(pronuclei rates) were not different significantly between every two groups.The 2 cell and 4 cell rates(59 26%,20 37%;63 79%,20 69%) in 10 and 20 mmol/L groups were higher significantly than those(29 63%,9 26%;47 06%,7 84%) in 1 6 and 5 0 mmol/L groups.Only minority(3 45%) in 20 mmol/L group reached morula stage.The activation rates of oocytes treated with 10 or 20 mmol/L SrCl 2 for 20 min were not different significantly,so did for 40 and 60 min treated groups.The morula and blastocyst rates(64 44%,22 22%) in the group that oocytes were treated with 20 mmol/L SrCl 2 for 40 min were higher significantly than those(30 36%,11 61%) with 10 mmol/L;but when the duration time prolong to 60 min,the morula and blastocyst rates(84 68%,46 77%) in the 10 mmol/L group were higher significantly than those(67 46%,28 57%) of 20 mmol/L group.In experiment 2,the effects of duration time of exposure to SrCl 2 on the parthenogenetic activation of mouse oocytes were studied.The activation rates and 2 cell rates were not different significantly for the oocytes treated with 10 mmol/L SrCl 2 for 20,40,60,180 and 360 min;the blastocyst rates(49 58% vs 47 95%) in 60 min group and 180 min group(treated in 10 mmol/L SrCl 2) were not different significantly,both were higher significantly than those(4 5%,12 24% and 35 53%) in 20,40 and 360 min group,the three latter differed significantly between every two groups.In experiment 3,the effect of oocyte age on oocyte parthenogenetic activation was studied.The activation rate(77 78%) of oocytes collected 16 h post hCG injection (treated with 10 mmol/L SrCl 2 for 60 min) was higher significantly than that(29 63%) of oocytes collected 14 h post hCG injection,and the activation rate(94 19%) of oocytes collected 18 h post hCG injection was higher significantly than that of oocytes collected at hCG 16 h. The blastocyst rates(6 71%,27 78%,47 67%) of oocytes collected at hCG 14,16,18 were different significantly between every two groups,the blastocyst rate(39 74%) of hCG 20 h was lower significantly than that of hCG 18 h.