土鳖虫粉对家兔高脂血症的预防及相关基因的调控

本研究以家兔为试验动物,建立高脂血症模型和预防给药模型,以探讨土鳖虫预防高脂血症的作用机理。试验周期为40 d,将试验动物分为空白对照组、高脂模型组和高、中、低3个剂量的预防给药组。预防给药组分别给予含不同剂量土鳖虫的饲料(0.4 g/kg、0.2 g/kg、0.1 g/kg)预防给药40 d。通过对家兔血清的血脂水平胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]进行测定;采用荧光定量PCR技术对各组家兔肝脏中载脂蛋白E基因(Apo E)、低密度脂蛋白受体基因(LDL-R)2个基因的表达量的变化进行研究。结果表明:土鳖虫粉可以调节血脂,对高脂血症有一定的预防作用,高剂量组的(0.4 g/kg)预防效果最好,中剂量(0.2 g/kg)次之,低剂量(0.1 g/kg)最差。

Prevention of Hyperlipidemia and Regulation of Related Geneby Eupolyphaga Seu Steleophaga Powder in Domestic Rabbit

A hyperlipidemia model was established with rabbit and used to study the prevention of hyperlipidemia with Eupolyphaga. The experiment lasted for 40 days and the rabbits were divide into five groups： high-fat group,blank group,the high,medium and low dosage of medicine treatment groups. Animals in prevention groups were fed a diet supplemeted with Eupolyphaga at 0. 4,0. 2,0. 1 g / kg for 40 days. The concentration of blood-lipoids（ TC,TG,LDL-C HDL-C） were determined and the expression of Apo E and LDL-R genes were measured by RT-PCR,thus the medical mechanism of Eupolyphaga was studied. The results of the experiment showed that Epolyphaga powder could regulate the level of blood liquid and prevent rabbits from the raising of blood liquid level. The data revealed that the preventive effect was dose dependent.