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Effects of salicylic acid on cold preservation and cryopreservation of encapsulated embryonic axes of Persian lilac (Melia azedarach L.)
Authors:F Bernard  H Shaker-Bazarnov  B Kaviani
Institution:(1) Plant Physiology and Biotechnology Laboratory, Shahid Beheshti University, Department of Biological Sciences, Evin, Tehran, I.R. of Iran
Abstract:Embryonic axes of Persian lilac (Melia azedarach L.)encapsulated into calcium alginate beads with sucrose (0.75 M) and salicylicacid (0, 50 mgrM, 200 mgrM) were subjected to cryopreservationtechnique with dehydration and freezing in liquid nitrogen or to coldpreservation by stocking alginate beads in empty petri dishes for 4 monthsat 4 °C. In these two cases 200 mgrM salicylic acid enhancedsignificantly the percentage of viability of encapsulated embryonic axes andthe role of salicylic acid in increasing tolerance to dehydration is discussed.
Keywords:cold preservation  cryopreservation  embryonic axes  Melia azedarach  encapsulation  salicylic acid
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