| 玉米花粉管通道法转化脱水素BDN1基因 |
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| 引用本文： | 孟宪玉,单长建,王乾坤,田雨,王振华,邸宏. 玉米花粉管通道法转化脱水素BDN1基因[J]. 玉米科学, 2014, 22(6): 32-35 |
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| 作者姓名： | 孟宪玉 单长建 王乾坤 田雨 王振华 邸宏 |
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| 作者单位： | 东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;黑龙江八一农垦大学/寒地作物种质改良与栽培重点实验室, 黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省留学归国人员科学基金(LC201411)、黑龙江省寒地作物种质改良与栽培重点实验室开放课题资助项目(CGIC201202) |
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| 摘 要： | 通过花粉管通道法将脱水素基因BDN1转入玉米自交系合344中,并对转化后代进行PCR和RT-PCR分子检测以及耐盐性功能鉴定,筛选耐盐性较高的转基因玉米新种质.结果表明,实验共获得88株T0代除草剂抗性植株,其中31株PCR检测呈阳性,14株RT-PCR检测呈阳性,对T4代转基因株系苗期进行300 mmol/L NaCl溶液的盐胁迫处理, 2个转基因株系耐盐性比对照提高两个级别.
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| 关 键 词： | 玉米 花粉管通道法 脱水素基因 BDN1 耐盐性 |
| 收稿时间： | 2013-09-12 |
Introduction of Dehydrin Gene(BDN1) into Maize by Pollen Tube Pathway |
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| MENG Xian-yu,SHAN Chang-jian,WANG Qian-kun,TIAN Yu,WANG Zhen-hua and DI Hong. Introduction of Dehydrin Gene(BDN1) into Maize by Pollen Tube Pathway[J]. Journal of Maize Sciences, 2014, 22(6): 32-35 |
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| Authors: | MENG Xian-yu SHAN Chang-jian WANG Qian-kun TIAN Yu WANG Zhen-hua DI Hong |
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| Affiliation: | Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions, Heilongjiang Bayi Agricultural University, Daqing 163319, China |
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| Abstract: | Dehydrin gene BDN1 was introduced into maize inbred line He344 by pollen tube pathway. Eighty-eight T0 maize tranformed plants resisted to herbicide, among which 31 plants showed positive PCR reaction and 14 plants showed positive RT-PCR reaction. Eight transgenic maize lines with BDN1 gene were identified salt-tolerance by observing the seedling appearance under 300 mmol/L NaCl stress. Two transgenic maize lines have improved their salt tolerance ability by two-level compared to acceptors. |
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| Keywords: | Maize Pollen tube pathway Dehydrin gene BDN1 Salt tolerance |
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