外引诱导系EMK-1诱导率和早熟玉米单倍体加倍技术研究

以3份加拿大早熟玉米种质为基础材料,利用外引俄罗斯诱导系EMK-1为父本进行人工诱导,通过子粒外观形态鉴定,确定准单倍体子粒.以加拿大早熟群体4诱导的单倍体植株为材料,设置秋水仙素浸种加倍、在6叶期秋水仙素注射加倍与自然加倍3个实验,对单倍体植株进行人工加倍.结果表明,3份加拿大早熟玉米种质单倍体诱导率存在很大差异,诱导率最高达14.37%,最低为5.82%.0.1%浓度的秋水仙素在植株6叶期注射植株加倍的处理效果最好,加倍率为26%;0.3%浓度的秋水仙素浸种12 h处理加倍效果次之,加倍率为14.63%;自然加倍加倍率为3.06%.

Research on the Induction Rate of EMK-1 and Doubling Method of Early Maturity Maize

Haploid materials of maize were gotten with EMK-1 from Russion as male parent and three early maturity maize materials from Canada as the base material. Doubling experiment with three methods were done, soaking seed in colchicine, injection colchicine at six leaf stage, natural doubling, which the haploid materials were gotten from the early maturity maize population of Canada No.4. The results indicated that haploid induction rates among different germplasm materials were different. The highest haploid induction rate was 14.37% while the lowest was 5.82%. The doubling result showed that the best method was the treatment with 0.1% colchicines at six leaf stage, the rates of 26%. The second was soaking seed in 0.3% colchicine for 12 h, the doubling rate was 14.63%, the natural doubling rate was only 3.06%.