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| 松毛虫AFLP反应体系的建立及引物筛选 | |
| 引用本文： | 王兴红,高宝嘉,南宫自艳,张映炳.松毛虫AFLP反应体系的建立及引物筛选[J].河北农业大学学报,2009,32(2). |
| 作者姓名： | 王兴红 高宝嘉 南宫自艳 张映炳 |
| 作者单位： | 1. 河北农业大学,林学院,河北,保定,071000 2. 河北农业大学,林学院,河北,保定,071000;河北北方学院,河北,张家口,075000 3. 湖南省洞口县森防站,湖南,邵阳,422300 |
| 摘 要： | 为研究不同环境条件下松毛虫的遗传多样性,以油松毛虫为材料研究松毛虫的AFLP反应体系。对选择性扩增中的预扩增产物稀释倍数、Mg2+浓度、dNTP浓度、引物浓度和Taq酶浓度进行了比较分析。结果显示,在选择性扩增体系中,预扩增产物稀释40倍,Mg2+浓度1.5 mmol/L、dNTP浓度0.2 mmol/L,引物浓度0.500pmol/L,Taq酶用量为1U,扩增产物经5%变性聚丙烯酰胺凝胶电泳可以得到稳定的结果。用优化的AFLP反应体系,以油松毛虫、赤松毛虫和马尾松毛虫为材料筛选引物,从81对引物组合中筛选出10对多态性高的引物组合。 |
| 关 键 词： | 松毛虫 AFLP 体系优化 引物筛选 |
Establishment of AFLP reaction system and screening of primer pairs for pine caterpillars |
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| WANG Xing-hong,GAO Bao-jia,NANGONG Zi-yan,ZHANG Ying-bing.Establishment of AFLP reaction system and screening of primer pairs for pine caterpillars[J].Journal of Agricultural University of Hebei,2009,32(2). | |
| Authors: | WANG Xing-hong GAO Bao-jia NANGONG Zi-yan ZHANG Ying-bing |
| Institution: | 1.College of Forestry;Agricultural University of Hebei;Baoding 071000;China;2.Hebei North University;Zhangjiakou 075000;3.Dongkou Forest Conservation Bureau of Hunan Province;Shaoyang 422300;China |
| Abstract: | To examine the genetic diversity of Dendrolimus tabulaeformis in different environment conditions,AFLP reaction system was optimized.In the selective amplification reaction,some important factors affecting the selective amplification were studied,including the dilute multiple of the pre-amplification production,Mg2+ concentration,dNTP concentration,primer concentration and the different dosage of Taq DNA polymerase.It was proved that the optimized system of AFLP for D.tabulaeformis was built: 40 dilute mult... |
| Keywords: | AFLP |
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