| 烟草蚀纹病毒弱株系的选育及免疫效果研究 |
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| 引用本文： | 张满良.烟草蚀纹病毒弱株系的选育及免疫效果研究[J].西北农林科技大学学报(社会科学版),1998,26(2):53-56. |
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| 作者姓名： | 张满良 |
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| 作者单位： | 西北农业大学植保系 |
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| 摘 要： | １９９１～１９９５年利用钴６０照射和亚硝酸处理进行了烟草蚀纹病毒（ＴＥＶ）弱株系的选择及防病效果试验。结果表明，钴６０照射病毒溶液能产生弱株突变体的适宜剂量是１．２９，２．５８Ｃ／ｋｇ，亚硝酸处理的适宜浓度和时间是１ｍｏｌ·Ｌ－１，３０～５０ｍｉｎ．经选择效果明显的弱株有Ｒ４和Ｈ３８，其室内交互保护试验的效果最高分别是８５．８％和４７．４％；田间防病效果分别为８１．６％和４０．３％．两个弱株免疫后接种强株，其烟株体内病毒含量均低于强株对照。试验还表明，亚硝酸处理较钴６０能产生较多的弱株突变体。
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| 关 键 词： | 烟草蚀纹病毒 钴60 亚硝酸 弱株系选择 |
Induced Mild Strains of Tobacco Etch Virus |
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| Zhang Manliang.Induced Mild Strains of Tobacco Etch Virus[J].Journal of Northwest Sci-Tech Univ of Agr and,1998,26(2):53-56. |
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| Authors: | Zhang Manliang |
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| Institution: | Zhang Manliang (Department of Plant Protection,Northwestern Agricultural University,Yangling,Shaanxi 712100) |
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| Abstract: | Mild strains of tobacco etch are studied.The suitable dosage of C060 treatment is 1. 29 C/kg and 2. 58 C/kg for producing TEV mutant. The suitable density of
HNO2 treatment is 1mol·L-1 30-50min.R4 strain and H38 strain are better.The protection effect of R4 and H38 is 85. 8% and 47. 4% in greenhouse, respectively.The effect
of R4 to control tobacco etch virus is 81. 6% in the field,where as H38 is 40. 3%.The virus
content of immune plant is lower than check.The results show that HNO2 treatment can
produce moremutants than C060. |
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| Keywords: | tobacco etch virus mild strain controlling virus effect |
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