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不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响
引用本文:韩忠燕,张宝红,黄海愉,张为宇,黄维义,钟恒禄. 不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响[J]. 中国畜牧兽医, 2009, 36(4): 100-102
作者姓名:韩忠燕  张宝红  黄海愉  张为宇  黄维义  钟恒禄
作者单位:(广西大学动物科学技术学院, 南宁 530005)
基金项目:广西自然科学基金,国家自然科学基金 
摘    要:为了探讨在离心洗涤水牛外周血单个核细胞(peripheral blood mononuclear cell, PBMC)过程中以移液器吹打法和晃动离心管法重悬细胞对其活率的影响。对经密度梯度离心后初步收集到的PBMC,分别用移液器吹打和晃动离心管2种方法重悬细胞,离心洗涤后对细胞活率进行计数。结果发现,移液器吹打法得到的细胞活率为(74.9±2.6)%,而晃动离心管法使细胞活率达到(98.5±0.5)%,差异极显著(P<0.01)。说明在洗涤PBMC时,晃动离心管法重悬细胞能大大提高细胞活率,可为那些对细胞活率要求高的试验提供一个较为有效的方法。

关 键 词:水牛外周血单个核细胞  重悬细胞法  细胞活率  

Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process
HAN Zhong-yan,ZHANG Bao-hong,HUANG Hai-yu,ZHANG Wei-yu,HUANG Wei-yi,ZHONG Heng-lu. Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(4): 100-102
Authors:HAN Zhong-yan  ZHANG Bao-hong  HUANG Hai-yu  ZHANG Wei-yu  HUANG Wei-yi  ZHONG Heng-lu
Affiliation:(College of Animal Science and Technology, Guangxi University, Nanning 530005, China)
Abstract:The peripheral blood mononuclear cell (PMBC) activity of buffalo was comported by different resuspension methods during washing process.The PBMC was separated by density gradient centrifugation,then cell was resuspended by transferpettor-spirting method & centrifuge tube-sloshing method, when cell was washed three times the activity was evaluated. The cell activity by transferpettor-spirting method & centrifuge tube-sloshing method was(74.9±2.6)% and(98.5±0.5)% respectively, the difference of cell activity was significant(P<0.01). The activity of PBMC was higher by centrifuge tube-sloshing, which could provide an useful method for further study that requiring higher cell activity. 
Keywords:buffalo peripheral blood mononuclear cell  resuspension methods  cell activity  
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