氮离子束与γ射线辐照日本晴和“9311”水稻突变体库的筛选

利用氮离子束和γ射线辐照处理粳稻日本晴和籼稻"9311",经过M1代损伤鉴定、M2代筛选和M3代重复鉴定,分别得到了740份和666份日本晴突变体,571份和781份"9311"突变体。获得的突变体发生了叶片、茎秆、穗部和籽粒、生理等性状的突变。在氮离子束辐照处理中,日本晴和"9311"分别在5.0×1016N+/cm2和2.5×1016N+/cm2剂量辐照时获得高突变率,为6.44%和6.38%;γ射线辐照处理中,2种材料均在150Gy剂量辐照时获得高突变率,分别为5.68%和9.44%。在本试验中,各高突变率群体均为辐照当代损伤最低的群体。新构建的突变体库将为水稻功能基因组学研究提供较好的基础材料。

THE SCREENING OF MUTANT LIBRARY FOR RICE VARIATIONS NIPPONBARE AND "9311" BY NITROGEN ION BEAM AND γ-RAYS IRRADIATION

The japonica rice cultivar "Nipponbare" and indica rice cultivar "9311" were treated by nitrogen ion beam implantation and γ-rays irradiation to induce mutants for genetic and functional genomics researches.The damage effects were evaluated in M_1 generation and M_1 plants were harvested by single plant.Screening in M_2 generation and identifying in M_3 generation,740 and 666 mutants in Nipponbare and 571 and 781 mutants in "9311",were induced by nitrogen ion beam and γ-rays.Morphological variations at vegetative and reproductive stages,including leaf,stalk,panicle,seed and various physiological characters,were commonly observed in the two mutagenized populations.The mutation frequency of 6.44% for Nipponbare at the ion beam dose of 5.0×10~(16) N~+/cm~2 and 6.38% for "9311" at 2.5×10~(16) N~+/cm~2 were observed,and for r-rays irradiation at dosage of 150Gy were 5.68% and 9.44% respectively.In the present study,the damages in M_1 generation of each higher mutation population were least.So,this new mutant library,which was obtained by ion beam implantation and γ-rays irradiation,may be helpful for gene identification and gene functional analysis in future.