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| 进境玉米中玉米内州萎蔫病菌的PCR检测 | |
| 引用本文： | 叶露飞,粟寒,周国梁,印丽萍,李孝军,杨赛军,易建平.进境玉米中玉米内州萎蔫病菌的PCR检测[J].植物病理学报,2014,44(2):121-128. |
| 作者姓名： | 叶露飞 粟寒 周国梁 印丽萍 李孝军 杨赛军 易建平 |
| 作者单位： | 舟山出入境检验检疫局, 浙江 316000; 江苏出入境检验检疫局, 南京 210001; 上海出入境检验检疫局, 上海 200135 |
| 基金项目： | 国家质检总局科研项目(2009IK259); 浙江省科技厅重大科技专项重点农业项目(2009c12054) |
| 摘 要： | 为了快速准确检测进境玉米样品中的玉米内州萎蔫病菌Clavibacter michiganensis subsp. nebraskensis(Cmn), 根据GenBank中Cmn的16S-23S序列设计引物CM1/CM4和引物PSM1/CM3。引物PSM1/CM3仅能从供试的4株Cmn菌株中扩增获得208 bp的预期产物, 而其他36株对照菌株均不能扩增出预期条带。灵敏度测试结果表明引物CM1/CM4和PSM1/CM3组合的巢式PCR方法的检测灵敏度高于常规PCR, 检测灵敏度可达40 fg DNA或6.8 CFU目标细菌。常规PCR和巢式PCR方法对进境美国玉米样品的阳性检出率分别为8%和24%, 试验结果表明所建立的PCR方法可用于玉米样品中Cmn的快速检测。 |
| 关 键 词： | 玉米 玉米内州萎蔫病菌 检测 PCR |
| 收稿时间： | 2013-01-06 |
Detection of Clavibacter michiganensis subsp. nebraskensis in imported corn by PCR |
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| YE Lu-fei,SU Han,ZHOU Guo-liang,YIN Li-ping,LI Xiao-jun,YANG Sai-jun,YI Jian-ping.Detection of Clavibacter michiganensis subsp. nebraskensis in imported corn by PCR[J].Acta Phytopathologica Sinica,2014,44(2):121-128. | |
| Authors: | YE Lu-fei SU Han ZHOU Guo-liang YIN Li-ping LI Xiao-jun YANG Sai-jun YI Jian-ping |
| Institution: | Zhoushan Inspection and Quarantine Bureau, Zhejiang 316000, China; Jiangsu Inspection and Quarantine Bureau, Nanjing 210001, China; Shanghai Inspection and Quarantine Bureau, Shanghai 200135, China |
| Abstract: | The primers CM1/CM4 and PSM1/CM3 were designed for specific and sensitive detection for Clavibacter michiganensis subsp. nebraskensis(Cmn). The primers PSM1/CM3 could amplify 4 strains of Cmn and get an expected 208 bp product, but no target band for other 36 strains of related species. The nested PCR by primers CM1/CM4 and PSM1/CM3 could detect as low as 40 fg DNA or 6.8 CFU bacteria of Cmn with a better sensitivity than conventional PCR with primers PSM1/CM3. The results of detection with 100 corn samples imported from USA showed 24% positive by nested PCR and 8% for conventional PCR. These detection methods for Cmn exhibited potential role in the routine detection for imported corn sample. |
| Keywords: | Clavibacter michiganensis |
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