应用免疫放射分析法鉴定水稻细菌性条斑病稻种

应用(125)~I-A蛋白与兔抗血清免疫球蛋白IgG非特异性结合原理,建立了检测水稻细菌性条斑病病原菌的免疫放射分析法。经反复检验表明:检测灵敏度约为10~3菌/ml,批内和批间变异参数(CV)均<15％,兔抗血清对其它病原细菌基本上无交叉反应。通过对我国南方6省部分水稻病区的331个品种稻种样品鉴定表明,水稻病区稻种自然带菌非常普遍,其阳性率高达81.3％。采用浓缩接种试验证明,免疫放射分析法应用于自然带菌稻种的鉴定是可行的。

THE APPLICATION OF IMMUNORADIOMETRIC ASSAY FOR RAPID IDENTIFICATION OF Xanthomonas campestris pv.oryzicola IN DISEASED RICE SEEDS

A rapid immunoradiometric assay (IRMA) for identification of Xant-homonas campestris pv.oryzicola has been established by the combination of 125I-protein A with serum immunoglobulin IgG from rabbits. This method for the determination of infected samples of rice, compared with many other traditional methods, is very simple.The experiments show that the sensitivity of IRMA is approximate 1000 germ cells/ml. The coefficient variation of intrabatch and interbatch (CV) is about 15%. The antiserum only has slight cross-reaction (4.7% ) against X.campestris pv. oryzae and other pathogenic bacteria of rice. Of 331 types of rice seeds chosen from infected fields of six provinces, 269 were positive, and the positive rate was as high as 81.3%, which indicated that most rice seeds in these areas were infected with the pathogenic bacteria. The identification of natural inoculated rice seeds by IRMA is effective and prove to be reliable by means of inoculation method.