初始接种量对四脊裸胞壳Dh菌株菌丝生物量的影响

在温度(27±1)℃和光照12L∶12D的条件下,用PD培养液对四脊裸胞壳(Emericella quadriclineata (Thom & Raper) Bejami)Dh菌株进行液体振荡培养(100 r/min),测定不同接种量和液体培养时间对菌丝生物量的影响.结果表明:将含干菌丝生物量为23.8 mg/mL的母液按1%~15%(V/V)的比例接种后,培养60 h以上,1%~15%各接种量处理均能产生21 mg/mL以上的菌丝生物量.通过逻辑斯蒂生长模型拟合,发现该菌在上述液培条件下的最大菌丝生物量为36.7 mg/mL左右.

Effect of amount of initial inoculation on mycelia biomass of Emericella quadriclineata(Thom & Raper) Bejami Dh

Effect of amount of initial inoculation on mycelia biomass of Emericella quadriclineata(Thom & Raper) Bejami Dh was investigated with shaking(100 r/min) culture of potato dextrose fluid at(27±1) ℃ and 12L∶12D.Seed culture containing 23.8 mg dry mycelia per milliliter was used to initiate 50 mL liquid culture at the proportions(V/V) of 1 %～15 %.After 60 h of incubation the cultures initiated 1 % to 15 % seed culture yielded a mycelial biomass higher than 21 mg/mL.The fitting of the data to Logistic growth model found that Emericella quadriclineata(Thom & Raper) Bejami Dh could produce a maximum biomass of 36.7 mg/mL at the above regime.