肾蕨和镰叶肾蕨配子体发育及孢子繁殖研究

 用无菌培养和常规繁殖方法培养肾蕨(Nephrolepis auriculata) 和镰叶肾蕨(N. falcata) 的孢子, 对其配子体发育过程进行形态学观察, 研究不同培养基中孢子的萌发率及成苗情况。结果表明, 两种肾蕨均具有孢子单裂缝、成熟配子体为对称的心形或阔心形、毛状体单细胞、性器较小等共同特征。培养基中无机盐的浓度对孢子萌发及配子体发育状况影响较大。分析了3种含盐量不同的改良Knopps固体培养基及基质培养基的优缺点, 以及培养基中无机盐浓度和蔗糖浓度对这两种植物配子体产生的影响, 表明蔗糖浓度低于2%利于孢子萌发。

Studies on Gametophyte Development and Spore Propagation of Nephrolepis auriculata and N. falcata

The spores of Nephrolepis auriculata and N. falcata were cultivated with sterile culture and routine propagation. The gametophyte development of the two species were observed morphologically. Spore germination rates and sporophyte appearance rates in various culture media and substrate were also studied.The results indicated the same characteristics of the gametophytes of the two species, such as the spores are all monolete, adult gametophytes are cordate or broad cordate, trichomes are unicellular and sex organ is small.The rates of spore germination and gametophyte development of the two specieswere influenced heavily by concentration ofmineral salt in various culture media. Advantages and disadvantages of the three improved Knoppssolid culture medium and substrate culture medium were analysed respectively, also the influence of mineral salt concentration in various culture medium and sucrose concentration on gametophytes of the two species.That indicates the sucrose concentration under 2% is good for spore germination.