| 寄生对菜粉蝶蛹血淋巴中血细胞和可溶性蛋白组份的影响 |
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| 引用本文： | 蔡峻,叶恭银,胡萃.寄生对菜粉蝶蛹血淋巴中血细胞和可溶性蛋白组份的影响[J].植物保护学报,2000,27(2):151-156. |
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| 作者姓名： | 蔡峻 叶恭银 胡萃 |
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| 作者单位： | 浙江大学植物保护系 |
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| 基金项目： | 国家自然科学基金(39770515) |
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| 摘 要： | 菜烩蝶Ｐｉｅｒｉｓ ｒａｐａｅ蛹被蝶蛹金小蜂Ｐｔｅｒｏｍａｌｕｓ ｐｕｐａｒｕｍ寄生后１～４ｄ，血细胞九、浆血细胞数、颗粒血细胞数和处于分裂期的浆血细胞数均显著高于同期未寄生蛹，而脂血九无明显差异。寄生后１２ｈ的寄生蛹与同期未寄生蛹的血淋巴可溶性蛋白浓度相差不大，而在寄生后１～４ｄ，寄生蛹的血淋巴可溶性蛋白浓度明显高于同期未寄生蛹。寄生蛹和未寄生蛹的血淋巴样品电泳凝胶扫描结果表明，寄生蛹和求学厅生
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| 关 键 词： | 蝶蛹金小蜂 菜粉蝶 血淋巴 血细胞 可溶性蛋白 |
EFFECT OF PARASITISM ON HFJMOCYTE POPULATION AND SOLUBLE PROTEIN COMPONENTS IN THE HEMOLYMPH OF PIERIS RAPAE PUPAE |
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| Cai Jun,Ye Gongyin and Hu Cui.EFFECT OF PARASITISM ON HFJMOCYTE POPULATION AND SOLUBLE PROTEIN COMPONENTS IN THE HEMOLYMPH OF PIERIS RAPAE PUPAE[J].Acta Phytophylacica Sinica,2000,27(2):151-156. |
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| Authors: | Cai Jun Ye Gongyin and Hu Cui |
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| Institution: | Department of Plant Protection, Zhejiang University, Hangzhou 310029,Department of Plant Protection, Zhejiang University, Hangzhou 310029 and Department of Plant Protection, Zhejiang University, Hangzhou 310029 |
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| Abstract: | The effect of parasitization by Pteromalus puparum on hemocyte population and soluble protein in the hemolymph of Pieris rapae pupae was investigated. It was found that, from 12 hours to the fourth day after parasitization, the total hemocyte counts(THC) were significantly higher in parasitized pupae than unparasitized ones, as well as the counts of plasmatocyte, granulocyte and plasmatocyte in mitosis, but the adipohemocyte count was not. The concentration of hemolymph soluble protein in parasitized pupae was similar with that in unparasitized pupae at 12 hours after parasitization, and then, markedly higher than the latter. The PAGE pattern revealed that the protein bands of hemolymph samples, from parasitized and the unparasitized pupae, were obviously different. |
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| Keywords: | Pteromalus pupanan Pieris rapae hemolymph hemocyte soluble protein |
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