| 超细型细毛羊基因组DNA的AFLP检测研究 |
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| 引用本文： | 贺三刚,黄锡霞,史洪才,田可川,徐新明,劳荃蘅.超细型细毛羊基因组DNA的AFLP检测研究[J].中国畜牧兽医,2008,35(7):32-36. |
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| 作者姓名： | 贺三刚 黄锡霞 史洪才 田可川 徐新明 劳荃蘅 |
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| 作者单位： | 1. 新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室，乌鲁木齐 830000；2.新疆畜牧科学院畜牧所，乌鲁木齐 830000； 3.新疆农业大学动物科学学院，乌鲁木齐 830052 |
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| 基金项目： | 新疆维吾尔自治区科技攻关
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重点项目计划项目 |
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| 摘 要： | 利用AFLP标记对超细型细毛羊品系纯度进行检测，旨在为评价该品系细毛羊的遗传特性，采用TaqⅠ和EcoRⅠ双酶切，利用6对引物对24只细毛羊基因组DNA进行分析，共获得226个AFLP标记，1对引物获得的标记数在29~50之间，新吉超细型细毛羊相似系数AFLP研究结果为0.914~0.982，本研究为评价新吉超细型细毛羊遗传稳定系数提供了相关的参数。
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| 关 键 词： | 超细型细毛羊 群体遗传结构 分子标记 AFLP |
Genetic Monitoring of Genomic DNA from Superfine Wool Sheep by AFLP |
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| HE San-gang,HUANG Xi-xia,SHI Hong-cai,TIAN Ke-chuan,XU Xin-ming,LAO Quan-heng.Genetic Monitoring of Genomic DNA from Superfine Wool Sheep by AFLP[J].China Animal Husbandry & Veterinary Medicine,2008,35(7):32-36. |
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| Authors: | HE San-gang HUANG Xi-xia SHI Hong-cai TIAN Ke-chuan XU Xin-ming LAO Quan-heng |
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| Institution: | 1. Xinjiang Academy of Animal Science， The Key Lab of Livestock Reproduction & Breed Biotechnology of MOA, Urumqi 830000,China;
2. Xinjiang Academy of Animal Science, Urumqi 830000，China； 3. College of Animal Science of Xinjiang Agricultural University, Urumqi 830052，China |
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| Abstract: | We reported the application of AFLP markers to detect genetic polymorphic loci Xinji superfine wool sheep and their amplified APLP results. Their genetic construction of the population was also analyzed. Six APLP primers were used, genomic DNA from 24 sheep were restrictive by TaqⅠand EcoRⅠ,226 AFLP markers were obtained, the obtain marker numbers of individual primer were between 29 and 50. The results indicated as the following: AFLP marker was suitable for analysis genetic polymorphism in sheep, and the similarity index of population in Xinji superfine wool sheep was 0.914 to 0.982.The study provideed an useful parameter with appraise genetic stability of Xinji superfine wool sheep. |
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| Keywords: | superfine merino sheep genetic construction of population molecular marker AFLP |
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