Response of potato cell cultures to aluminium and selection of tolerant cell lines |
| |
| Authors: | G Wersuhn H H Nhi E Tellhelm T Börner |
| |
| Institution: | (1) Department of Genetics, Section of Biology, Humboldt-University, Invalidenstrasse 43, 1040 Berlin, DDR;(2) Institute of Potato Research, Academy of Agricultural Sciences of the GDR, 2551 Gross Lüsewitz, DDR |
| |
| Abstract: | Summary We developed a method for the selection of aluminium-tolerant cell lines by using a liquid medium which more closely simulates
acid soil conditions than media used previously. This medium has a pH of 4.8 and contains Al2(SO4)3 instead of Al-EDTA as the selective agent to avoid the toxic effects of EDTA. It is shown that Al2(SO4)3 exhibits a similar toxic effect on the growth of intact plants and cultured cells. With this medium, potato cell lines tolerating
2 mmol/l aluminium have been selected. |
| |
| Keywords: | Al-EDTA pH liquid medium aluminum sulphate intact plants Solanum tuberosum L |
| 本文献已被 SpringerLink 等数据库收录！ |
