| 大米草Na~+/H~+逆转运蛋白基因片段的克隆(英文) |
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| 引用本文： | 兰涛,段远霖,吴为人,汪斌,周元昌.大米草Na~+/H~+逆转运蛋白基因片段的克隆(英文)[J].福建农林大学学报(自然科学版),2006,35(1):52-56. |
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| 作者姓名： | 兰涛 段远霖 吴为人 汪斌 周元昌 |
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| 作者单位： | 1. 福建农林大学作物科学学院
2. 福建农林大学生命科学学院,福建,福州,350002 |
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| 摘 要： | 根据水稻的保守区设计引物,利用PCR体外扩增技术,从大米草中克隆Na+/H+逆转运蛋白基因片段.克隆出了一段长度为862 bp的DNA序列,结果表明它与水稻、大麦、小麦、棉花、拟南芥等植物的Na+/H+逆转运蛋白基因序列具有很高的同源性,证明该序列确属Na+/H+逆转运蛋白基因的部分片段.
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| 关 键 词： | 大米草 Na+/H+逆转运蛋白基因 克隆 |
| 文章编号： | 1671-5470(2006)01-0052-05 |
| 收稿时间： | 2004-11-28 |
| 修稿时间： | 2005-09-15 |
Cloning of a segment of Na+/H+ antiporter gene from Spartina anglica |
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| LAN Tao,DUAN Yuan-lin,WU Wei-ren,WANG Bin,ZHOU Yuan-chang.Cloning of a segment of Na+/H+ antiporter gene from Spartina anglica[J].Journal of Fujian Agricultural and Forestry University,2006,35(1):52-56. |
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| Authors: | LAN Tao DUAN Yuan-lin WU Wei-ren WANG Bin ZHOU Yuan-chang |
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| Institution: | 1. College of Crop Science; 2. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | A segment of Na~ /H~ antiporter gene from English cordgrass was cloned by PCR using a pair of primers designed according to the conserved domains of rice.A sequence of 862 bp in length was obtained.The sequence had high homologies to the Na~ /H~ antiporter genes from rice,barley,wheat,cotton,Arabidopsis and other plants.The result showed that it was a part of the Na~ /H~ antiporter gene from English cordgrass. |
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| Keywords: | English cordgrass Na /H antiporter gene cloning |
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