豆渣酶解工艺及其水解液发酵抗性菌株筛选

从工业化生产实际出发,探讨了豆渣的高效水解技术、酵母菌株的选育及其培养工艺。不同工艺条件下,采用纤维素酶和普鲁兰酶水解豆渣,以葡萄糖为标准测量水解后料液中糖含量,得出水解豆渣的最佳工艺条件:温度55℃,料液比为1∶24,先用普鲁兰酶水解,调至pH5.0,再用纤维素酶水解,调至pH5.5,普鲁兰酶3 h,纤维素酶1 h,时间比为3∶1,加酶总量为3%,普鲁兰酶∶纤维素酶为3∶1。用此条件对豆渣进行水解得到水解液,料液中含糖量最高位为160.7 mg·kg~(-1)。用含量为35%的水解液去培养抗性菌株,筛选的抗性菌株C发酵效果较好,酵母含量达18.49 g·L~(-1)。

Hydrolysis Technology of Bean Dregs and the Hydrolysis Resistant Strains Screening

Based on industrial production practice,this study discussed the efficient hydrolysis technology of bean dregs,breeding of yeast strains and high density cultivation process.Cellulase and prandlase hydrolyzed soybean residue were used in different technological conditions,the amount of sugar in the liquid was measured with glucose as the standard.The optimum technological conditions of hydrolyzed soybean residue were obtained as follows:55℃,solid to liquid ratio 1∶24,hydrolysis firstly with pullulanase,adjusted to pH5.0,hydrolysis followed with cellulase,adjusted to pH5.5,hydrolysis 3 h with pullulanase and 1 h with cellulase,the time ratio was 3∶ 1,enzyme amount was 3％,enzyme ratio 1∶1,pullulanase∶ cellulase =3∶1.This condition was used to hydrolyze soybean residue,the content of the material liquid contained the highest content of 160.7 mg· kg-1.Material liquid amount of 35％ were used to culture resistant strain,the content of C yeast showed high resistance,contented yeast of 18 g·L-1.