大豆疫霉根腐病菌游动孢子侵染野生大豆下胚轴的细胞学观察

用大豆疫霉根腐病菌的游动孢子悬浮液处理抗感不同野生大豆下胚轴,在接种后1.5~24 h,使用扫描电镜观察了野生大豆与大豆疫霉菌的亲和性和非亲和性互作。结果显示:接种1.5 h后游动孢子在感病野生大豆下胚轴表面产生休止孢,并产生芽管;接种3 h后休止孢萌发产生附着孢,开始侵入下胚轴表皮;接种6 h后侵入加深;接种12 h后附着孢完全进入下胚轴表皮;接种24 h后附着孢孢膜已经开始进入到表皮里。游动孢子悬浮液处理抗病野生大豆后,其侵染过程与亲和反应相似,只是游动孢子在下胚轴表皮产生芽管时间和附着孢侵入下胚轴表皮层的时间比亲和反应迟。

Cytological Observation on Infection Process of Hypocotyls of Wild Soybeans by Zoospore of Phytophthora sojae

The compatible and incompatible interactions between susceptible and resistant wild soybeans with zoospores of Phytophthora sojae race 1,the predominant race in Heilongjiang province at present were examined by electronmicroscopy,after inoculation from 1.5 to 24 h.The agerminating cysts were formed on hypocotyls of susceptible wild soybean at 1.5 h after inoculation,and appressoria began to penetrate into epidermis at 3 h,then totally into epidermis at 12 h.The appressoria membrane infected into epidermis at 24 h after inoculation.The infection process in incompatible interactions was similar to that in compatible interaction.However,the process of incompatible interactions was relatively late compared to that of compatible interaction.