Evaluation of a recombinant measles virus expressing hepatitis C virus envelope proteins by infection of human PBL-NOD/Scid/Jak3null mouse |
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| Authors: | Masaaki Satoh Makoto Saito Kohsuke Tanaka Sumako Iwanaga Salem Nagla Elwy Salem Ali Takahiro Seki Seiji Okada Michinori Kohara Shinji Harada Chieko Kai Kyoko Tsukiyama-Kohara |
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| Institution: | a Department of Experimental Phylaxiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto-city, Kumamoto 860-8556, Japan;b Division of Hematopoiesis, Center for AIDS Research, Kumamoto University, Japan;c Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Japan;d Department of Medical Virology, Faculty of Life Sciences, Kumamoto University, Japan;e Laboratory of Animal Research Center, Institute of Medical Science, University of Tokyo, Japan |
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| Abstract: | In this study, we infected NOD/Scid/Jak3null mice engrafted human peripheral blood leukocytes (hu-PBL-NOJ) with measles virus Edmonston B strain (MV-Edm) expressing hepatitis C virus (HCV) envelope proteins (rMV-E1E2) to evaluate the immunogenicity as a vaccine candidate. Although human leukocytes could be isolated from the spleen of mock-infected mice during the 2-weeks experiment, the proportion of engrafted human leukocytes in mice infected with MV (103–105 pfu) or rMV-E1E2 (104 pfu) was decreased. Viral infection of the splenocytes was confirmed by the development of cytopathic effects (CPEs) in co-cultures of splenocytes and B95a cells and verified using RT-PCR. Finally, human antibodies against MV were more frequently observed than E2-specific antibodies in serum from mice infected with a low dose of virus (MV, 100–101 pfu, and rMV-E1E2, 101–102 pfu). These results showed the possibility of hu-PBL-NOJ mice for the evaluation of the immunogenicity of viral proteins. |
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| Keywords: | MV HCV E1 E2 Human PBL NOD/Scid/Jak3null mouse |
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