| 鸡蛋黄抗体(IgY)的分离与研究 |
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| 引用本文： | 刘华,黄宗洲,程金科.鸡蛋黄抗体(IgY)的分离与研究[J].北京农学院学报,1991(2). |
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| 作者姓名： | 刘华 黄宗洲 程金科 |
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| 作者单位： | 北京农学院牧医系
(刘华，黄宗洲)，北京农学院牧医系(程金科) |
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| 摘 要： | 以鸡白痢沙门氏菌灭活苗,鸡新城疫(NDV)油剂灭活苗,传染性法氏囊炎(IBDV)油剂灭活苗,分别免疫接种健康母鸡.用异丙醇——丙酮法,分离提取高免鸡鸡蛋的蛋黄抗体(IgY)·经凝集试验,血球凝集抑制试验(HI)和琼脂扩散试验(AGP)检测蛋黄抗体(IgY)的抗体滴度,结果分别均高于血清的抗体滴度.另外,还以聚丙烯酰胺凝胶电泳(SDS-PAGE)监测比较了蛋黄抗体(IgY),血清和蛋黄液,结果表明,IgY的抗体球蛋白纯度均高于血清和蛋黄液.
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| 关 键 词： | 蛋黄抗体(IgY) 异丙醇-丙酮 |
STUDIES ON ISOLATION IgY ANTIBODIES IN IMMUNIZED HENS |
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| Liu Hua,Huang Zongzhou,Cheng Jinke Beijing Agricultual College.STUDIES ON ISOLATION IgY ANTIBODIES IN IMMUNIZED HENS[J].Journal of Beijing Agricultural College,1991(2). |
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| Authors: | Liu Hua Huang Zongzhou Cheng Jinke Beijing Agricultual College |
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| Institution: | Liu Hua;Huang Zongzhou;Cheng Jinke Beijing Agricultual College |
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| Abstract: | A procedure of 2-propanol and actone was developed for the isolation and purification of IgY antibodies from egg yolk of hens which were immunized with vaccine of Salmonella Pullorum or NDV or IBDV. The titres of IgY antibody appear to have higher than serum by test of agglutination or HI or AGP. The purity of immunoglobalins is higher than serum and yolk which were determined by SDS-PAGE. |
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| Keywords: | IgY antibody 2-propanol and actone |
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