转Bt基因玉米幼苗残体中Cry1Ab杀虫蛋白田间降解动态 |
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引用本文: | 邢珍娟,王振营,何康来,白树雄. 转Bt基因玉米幼苗残体中Cry1Ab杀虫蛋白田间降解动态[J]. 中国农业科学, 2008, 41(2): 412-416. DOI: 10.3864/j.issn.0578-1752.2008.02.013 |
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作者姓名: | 邢珍娟 王振营 何康来 白树雄 |
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作者单位: | 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室 |
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摘 要: | 【目的】研究间苗后留在田间地表的转Bt基因玉米幼苗残体中Cry1Ab杀虫蛋白的降解规律,比较两种Bt玉米幼苗残体中Cry1Ab杀虫蛋白的降解速度。【方法】以两种表达Cry1Ab杀虫蛋白的转Bt基因抗虫玉米MON810和Bt11为材料,采用ELISA方法测定各取样时期中幼苗残体中Cry1Ab杀虫蛋白残留量。【结果】转Bt基因玉米幼苗残体中杀虫蛋白降解是逐渐的,且降解速度较快,到50 d时幼苗残体已经完全腐烂,Bt11幼苗残体中的杀虫蛋白已经完全降解,在MON810中还能检测到微量的杀虫蛋白。两种转基因玉米幼苗残体中的Bt杀虫蛋白的初始含量差异不显著,但在同一时间段的Bt杀虫蛋白降解速度存在差异均显著,在30 d前MON810幼苗残体中Bt杀虫蛋白降解速度比Bt11降解的快,30d后,则降解趋势相反,到50 d取样结束时MON810和Bt11分别降解了初始含量的99.81%和100%。【结论】两种转Bt基因玉米间苗后留在田间的幼苗残体中的Cry1Ab杀虫蛋白降解速度不同,在50 d完全腐烂时,其中的杀虫蛋白完全降解或仅有微量残留。
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关 键 词: | 转Bt基因玉米 幼苗残体 Cry1Ab杀虫蛋白 降解 |
收稿时间: | 2006-09-12 |
Degradation Dynamics of Cry1Ab insecticidal protein within transgenic Bacillus thuringiensis corn seedling debris in field |
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Abstract: | Degradation Dynamics of Cry1Ab insecticidal protein within transgenic Bacillus thuringiensis corn seedling debris in fieldXing Zhen-juan, Wang Zhen-ying*, He Kang-lai, Bai Shu-xiong(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)Abstract: The degradation dynamics of Cry1Ab insecticidal protein within transgenic Bacillus thuringiensis corn seedling debris of events MON810 and Bt11 in field were investigated and compared..Results showed that the degradation of Cry1Ab toxin in transgenic corn seedlings were gradual and degraded rapidly The Cry1Ab insecticidal protein in Bt11 corn seedling debris could not been detected, but small amount of Cry1Ab toxin in MON810 could still be detected when the corn seedling decayed completely 50 days after picked off, The speed of degradation were significantly different within the same time between the two events. The Cry1Ab insecticidal protein in corn seedling debris of events Bt 11 was slower than that in MON810 within the 30 days, but faster than that in MON810 after 30 days. The Cry1Ab insecticidal protein in events MON810 and Bt11 decreased to 99.81 and 100 of the initial amount, respectively. |
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