沙漠微生物群落功能多样性分析

以宁夏回族自治区沙坡头地区的沙漠微生物群落为研究对象,采用Biolog方法研究了已固定沙丘结皮(J1)及结皮下层(J2),半流动沙丘上层(L1)和下层(L2)中微生物群落的功能多样性。结果表明,J1的微生物群落活性最高,J2的微生物群落次之,再次为L2的微生物群落,最后为L1的微生物群落;J1,J2,L1和L2的微生物群落对羧酸类、聚合物类和氨基酸类碳源的利用表现一致,对糖类、胺/氨类和双亲化合物类碳源的利用表现不同;利用ECO板上31种碳源进行主成分分析得出,PC1,PC2和PC3特征值的贡献率为分别为70.16%,19.23%,10.61%。研究结果表明:(1)经过固沙后的微生物群落活性明显高于半流动沙漠微生物群落的活性;(2)微生物群落结构上的差异致使其对6类碳源的利用方式不同;(3)主成分分析表明沙漠微生物群落对羧酸类、糖类和氨基酸类碳源的利用最高,主要利用碳源有22种。

An Analysis on Diversity of Microbial Community Function in Desert

Taking the microbial communities in the Shapotou deserted area of Ningxia Hui Autonomous Region as a research object,function diversity of microbial communities from fixed dunes crust(J1),sub fixed dunes crust(J2),semi-mobile dunes(L1) and sub semi-mobile dunes(L2) were studied using Biolog method.Results showed that physiological action of J1 microbial community was the highest,followed by J2,L2 and L1 in turn.J1,J2,L1 and L2 had the same performance in utilization of carboxylic acids,polymers and amino acid,but were different in utilization of sugar,amine/ammonia and parent compounds.The characteristic value contributions of PC1,PC2 and PC3 were 70.16%,19.23% and 10.61%,respectively.It can be concluded that(1) microbial community activity in fixed desert was significantly higher than that in mobile desert;(2) the differences of microbial community structure may result in the utilization of six types of carbon sources in different ways;(3) by principal component analysis,the utilization of acid,sugar and amino acid by desert microbial community was highest and carbon sources utilized were as many as 22 types.