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Detection of potato spindle tuber viroid by molecular hybridization and bioassay. A large-scale comparison |
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| Authors: | Marek Wełnicki Jacek L. Skrzeczkowski Włodzimierz Zagorski Stanisława Skrzeczkowska Anna Kowalska-Noordam Mirosława Was Waldemar Marczewski |
| Affiliation: | (1) Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Rakowiecka 36 str., 02-532 Warsaw, Poland;(2) Biotechnology Division of Trade Center of Polish Academy of Sciences, Miodowa 2 str., 00-080 Warsaw, Poland;(3) Potato Research Institute, Młochów, 05-832 Rozalin, Poland |
| Abstract: | Summary To assess the reliability of dot-spot hybridization assay for the Potato Spindle Tuber Viroid, more than 1000 potato samples were cross-tested by biological and hybridization methods. Both methods gave similar results with a range of cultivars. The dot-spot hybridization test was reproducible with formaldehyde-denaturated cell-sap samples stored at room temperature for over 6 months. This finding means that samples can be transported from field stations to a diagnostic laboratory and allows for cross-checking of results between laboratories. The data suggest that dot-spot hybridization is suitable for breeding and quarantine purposes. |
| Keywords: | DNA probe nick-translation diagnosis Solanum tuberosum L. |
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