| 毒死蜱对舞毒蛾食物利用和解毒酶及AChE活性的影响 |
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| 引用本文： | 李慧,严善春,王志英,葛士林,曹传旺. 毒死蜱对舞毒蛾食物利用和解毒酶及AChE活性的影响[J]. 林业科学研究, 2011, 24(3): 345-349 |
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| 作者姓名： | 李慧 严善春 王志英 葛士林 曹传旺 |
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| 作者单位： | 1. 东北林业大学林学院森林保护学科,黑龙江哈尔滨,150040
2. 东北林业大学林学院森林保护学科,黑龙江哈尔滨150040;东北林业大学林木遗传育种与生物技术教育部重点实验室,黑龙江哈尔滨150040 |
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| 基金项目： | 国家973计划前期研究专项(2009CB125906);国家公益性行业科研项目(200904029-4);中国博士后基金(20090450942) |
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| 摘 要： | 采用质量法和酶活性测定法研究了毒死蜱对舞毒蛾(Lymantria dispar)3龄幼虫食物利用的影响,并测定了其毒力及解毒酶、乙酰胆碱酯酶(AChE)的活性。用亚致死浓度(1.5 mg·L-1)毒死蜱处理小黑杨叶片,饲喂舞毒蛾3龄幼虫,其幼虫生长率(RGR)、食物利用率(ECI)和食物转化率(ECD)均显著低于对照,而近似消化率(AD)显著高于对照,相对取食量(RCR)处理和对照间差异不显著。毒死蜱对舞毒蛾幼虫24 h致死中浓度(LC50)为5.86 mg·L-1,其毒力低于三氟氯氰菊酯而高于氧化乐果。毒死蜱对舞毒蛾3龄幼虫体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和AChE均有抑制作用,抑制程度为CarE＞AChE＞GSTs。毒死蜱通过影响舞毒蛾食物利用和抑制酶活性而表现出杀虫活性,为一种有效防治舞毒蛾的有机磷杀虫剂。
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| 关 键 词： | 毒死蜱 舞毒蛾 食物利用 解毒酶 乙酰胆碱酯酶 |
| 收稿时间： | 2010-01-07 |
Effects of Chlorpyrifos on Food Utilization and Detoxifying Enzymes and Acetylcholinesterase of Lymantria dispar |
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| LI Hui,YAN Shan-chun,WANG Zhi-ying,GE Shi-lin and CAO Chuan-wang. Effects of Chlorpyrifos on Food Utilization and Detoxifying Enzymes and Acetylcholinesterase of Lymantria dispar[J]. Forest Research, 2011, 24(3): 345-349 |
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| Authors: | LI Hui YAN Shan-chun WANG Zhi-ying GE Shi-lin CAO Chuan-wang |
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| Affiliation: | Department of Forestry Protection, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Department of Forestry Protection, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, Harbin 150040, Heilongjiang, C;Department of Forestry Protection, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, Harbin 150040, Heilongjiang, C;Department of Forestry Protection, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Department of Forestry Protection, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, Harbin 150040, Heilongjiang, C |
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| Abstract: | The effects of sublethal dose of chlorpyrifos(CPF) on food utilization of gypsy moth,Lymantria dispar,as well as CPF toxicity and enzyme activities of carboxylesterase(CarE),glutathione S-transferases(GSTs) and acetylcholinesterase(AChE) were evaluated in the 3rd-instar gypsy moth larvae using gravimetric method and measuring enzyme activities.Relative growth rate(RGR),efficiency of the conversion of ingested food(ECI) and efficiency of the conversion of digested food(ECD) of 3rd-instar larvae fed on poplar... |
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| Keywords: | chlorpyrifos gypsy moth food utilization detoxifying enzymes AChE |
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