| 离体昆虫细胞对 AcNPV和BmNPV的敏感性测定与分析 |
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| 引用本文： | 张欣,冯颖,丁伟峰,马涛.离体昆虫细胞对 AcNPV和BmNPV的敏感性测定与分析[J].林业科学研究,2011,24(3):321-326. |
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| 作者姓名： | 张欣 冯颖 丁伟峰 马涛 |
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| 作者单位： | 中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224 |
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| 基金项目： | 林业公益性行业科研专项(编号:4-38) |
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| 摘 要： | 采用来源于3个目14种昆虫的离体细胞系对苜蓿银纹夜蛾核型多角体病毒AcNPV(Autographa californica nuclear polyhedrosis virus)和家蚕核型多角体病毒BmNPV(Bombyx mori nuclear polyhedrosis virus)进行敏感性测定.试验结果表明:各...
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| 关 键 词： | 昆虫细胞系 AcNPV BmNPV 感染 |
| 收稿时间： | 2011/1/10 0:00:00 |
Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV |
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| ZHANG Xin,FENG Ying,DING Wei-feng and MA Tao.Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV[J].Forest Research,2011,24(3):321-326. |
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| Authors: | ZHANG Xin FENG Ying DING Wei-feng and MA Tao |
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| Institution: | Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Kunming 650224, Yunnan, China |
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| Abstract: | Fourteen insect cell lines from three orders were used to be infected with Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori nuclear polyhedrosis virus (BmNPV). The result showed that the susceptibility for two viruses were different among different insect cell lines. The cell lines which come from Diptera and Orthoptera could not be infected with AcNPV and BmNPV. The five cell lines, Sf21, Sf9, HighFive, RIRI-PX and HZ, which come from Lepidoptera, could be infected with AcNPV. The BmN cell line could be infected only with BmNPV. The infection rate of AcNPV to Sf9 was the highest, reached 84.0% after 10 days infection. RIRI-PX infected with AcNPV could produce the highest yield of polyhedral inclusion bodies which reached 43.9 PIBs/cell at 10 day post infection. The infection rate of BmNPV to BmN was 72.2% after 10 days infection, and the yield of polyhedral inclusion bodies was 23.1PIBs/cell. |
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| Keywords: | insect cell lines AcNPV BmNPV infection |
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